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- W1673886354 abstract "A high performance liquid chromatographic (HPLC) method, based on dansylation and fluorescence detection, is described for the estimation of putrescine, spermidine, and spermine in lichen (Evernia prunastri [L.]) samples. Because of the high concentrations of phenols and salts, dansylation was followed by a pre-HPLC purification step. Both flow rate and mobile phase (methanol:water) followed a gradient for optimum resolution on a reverse-phase column. Amounts as small as 0.3 picomole of standard polyamines could be detected. In applying the method to lichens, it was found that 5.45% (w/w) of the exogenous putrescine taken up by the thallus was unbound in the algal partner and that 60% (w/w) was conjugated in the thallus, perhaps to lichen phenolics." @default.
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- W1673886354 date "1988-06-01" @default.
- W1673886354 modified "2023-10-16" @default.
- W1673886354 title "High Performance Liquid Chromatography of the Dansyl Derivatives of Putrescine, Spermidine, and Spermine" @default.
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- W1673886354 doi "https://doi.org/10.1104/pp.87.2.519" @default.
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