Matches in SemOpenAlex for { <https://semopenalex.org/work/W1674509114> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1674509114 abstract "A binding protein for sn-glycerol 3-phosphate was isolated from the cell envelope of Escherichia coli by the cold osmotic shock procedure. The protein was purified to homogeneity. It has a molecular weight of 45,000 and binds sn-glycerol 3-phosphate with a KD of 0.2 microM. The protein is monomeric and has L-leucine as NH2-terminal amino acid. The intrinsic fluorescence of the protein is altered upon binding of substrate. At an excitation of 285 nm, the emission maximum at 340 nm is quenched and shifted to 330 nm. Binding of sn-glycerol 3-phosphate is reversible and no chemical alteration occurs with the substrate. The appearance of the binding protein in the periplasm is the result of a mutation that renders the cells constitutive for sn-glycerol 3-phosphate transport. Simultaneously, two other proteins appear in the periplasm. These proteins were also purified. They do not bind sn-glycerol 3-phosphate and do not cross-react with antibodies against the pure binding protein." @default.
- W1674509114 created "2016-06-24" @default.
- W1674509114 creator A5002278792 @default.
- W1674509114 creator A5004902921 @default.
- W1674509114 date "1979-11-01" @default.
- W1674509114 modified "2023-09-29" @default.
- W1674509114 title "Purification and properties of the sn-glycerol 3-phosphate-binding protein of Escherichia coli." @default.
- W1674509114 cites W1484190324 @default.
- W1674509114 cites W1495603474 @default.
- W1674509114 cites W1511524557 @default.
- W1674509114 cites W1532724693 @default.
- W1674509114 cites W1535979494 @default.
- W1674509114 cites W1543585074 @default.
- W1674509114 cites W1574118979 @default.
- W1674509114 cites W1599579794 @default.
- W1674509114 cites W1605288412 @default.
- W1674509114 cites W1620838672 @default.
- W1674509114 cites W1692475556 @default.
- W1674509114 cites W1708448089 @default.
- W1674509114 cites W1878379466 @default.
- W1674509114 cites W1982433122 @default.
- W1674509114 cites W2008166760 @default.
- W1674509114 cites W2027393724 @default.
- W1674509114 cites W2054504350 @default.
- W1674509114 cites W2065497078 @default.
- W1674509114 cites W2100837269 @default.
- W1674509114 cites W2106215590 @default.
- W1674509114 cites W2157787228 @default.
- W1674509114 cites W2177399548 @default.
- W1674509114 cites W4254907307 @default.
- W1674509114 cites W7832712 @default.
- W1674509114 cites W997127808 @default.
- W1674509114 doi "https://doi.org/10.1016/s0021-9258(19)86613-9" @default.
- W1674509114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/387762" @default.
- W1674509114 hasPublicationYear "1979" @default.
- W1674509114 type Work @default.
- W1674509114 sameAs 1674509114 @default.
- W1674509114 citedByCount "34" @default.
- W1674509114 countsByYear W16745091142013 @default.
- W1674509114 countsByYear W16745091142021 @default.
- W1674509114 countsByYear W16745091142022 @default.
- W1674509114 crossrefType "journal-article" @default.
- W1674509114 hasAuthorship W1674509114A5002278792 @default.
- W1674509114 hasAuthorship W1674509114A5004902921 @default.
- W1674509114 hasBestOaLocation W16745091141 @default.
- W1674509114 hasConcept C104317684 @default.
- W1674509114 hasConcept C185592680 @default.
- W1674509114 hasConcept C18903297 @default.
- W1674509114 hasConcept C201663137 @default.
- W1674509114 hasConcept C2777132085 @default.
- W1674509114 hasConcept C2777289219 @default.
- W1674509114 hasConcept C2780881558 @default.
- W1674509114 hasConcept C46371251 @default.
- W1674509114 hasConcept C515207424 @default.
- W1674509114 hasConcept C547475151 @default.
- W1674509114 hasConcept C553089730 @default.
- W1674509114 hasConcept C55493867 @default.
- W1674509114 hasConcept C86803240 @default.
- W1674509114 hasConceptScore W1674509114C104317684 @default.
- W1674509114 hasConceptScore W1674509114C185592680 @default.
- W1674509114 hasConceptScore W1674509114C18903297 @default.
- W1674509114 hasConceptScore W1674509114C201663137 @default.
- W1674509114 hasConceptScore W1674509114C2777132085 @default.
- W1674509114 hasConceptScore W1674509114C2777289219 @default.
- W1674509114 hasConceptScore W1674509114C2780881558 @default.
- W1674509114 hasConceptScore W1674509114C46371251 @default.
- W1674509114 hasConceptScore W1674509114C515207424 @default.
- W1674509114 hasConceptScore W1674509114C547475151 @default.
- W1674509114 hasConceptScore W1674509114C553089730 @default.
- W1674509114 hasConceptScore W1674509114C55493867 @default.
- W1674509114 hasConceptScore W1674509114C86803240 @default.
- W1674509114 hasLocation W16745091141 @default.
- W1674509114 hasOpenAccess W1674509114 @default.
- W1674509114 hasPrimaryLocation W16745091141 @default.
- W1674509114 hasRelatedWork W1495603474 @default.
- W1674509114 hasRelatedWork W1499122119 @default.
- W1674509114 hasRelatedWork W1514310691 @default.
- W1674509114 hasRelatedWork W1532724693 @default.
- W1674509114 hasRelatedWork W1546783991 @default.
- W1674509114 hasRelatedWork W1582951957 @default.
- W1674509114 hasRelatedWork W1597626888 @default.
- W1674509114 hasRelatedWork W1775749144 @default.
- W1674509114 hasRelatedWork W1899568910 @default.
- W1674509114 hasRelatedWork W1945398681 @default.
- W1674509114 hasRelatedWork W1973719474 @default.
- W1674509114 hasRelatedWork W1994800767 @default.
- W1674509114 hasRelatedWork W2009708970 @default.
- W1674509114 hasRelatedWork W2042814467 @default.
- W1674509114 hasRelatedWork W2057481976 @default.
- W1674509114 hasRelatedWork W2100837269 @default.
- W1674509114 hasRelatedWork W2105012761 @default.
- W1674509114 hasRelatedWork W2131948396 @default.
- W1674509114 hasRelatedWork W2180739100 @default.
- W1674509114 hasRelatedWork W997127808 @default.
- W1674509114 isParatext "false" @default.
- W1674509114 isRetracted "false" @default.
- W1674509114 magId "1674509114" @default.
- W1674509114 workType "article" @default.