FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1676059852> ?p ?o ?g. }

• W1676059852 endingPage "1217" @default.
• W1676059852 startingPage "1212" @default.
• W1676059852 abstract "Abstract Many mAb that bind the carbohydrate antigenic determinant 3-fucosyl-lactosamine (3-FL), Gal beta 1-4[Fuc alpha-3]GlcNAc-R have been raised in BALB/c mice, and we are studying the structure and regulation of these antibodies. In this report, we present the first information about their amino acid sequences and the Ig gene segments used to encode them. V regions of the H and L chains of three anti-3-FL antibodies, PMN6, PMN29, and PM81, were sequenced by a combination of mRNA and amino acid sequencing. The L chain sequences of PMN6 and PM81 antibodies indicate that their VK and JK regions are encoded by VK24B and JK1 germ-line genes, respectively. The nucleotide and amino acid sequences of the H chains suggest that the three anti-3-FL antibodies are encoded by the VH441 gene segment of the X24 VH family, and this conclusion was supported by Southern filter hybridization with VH441 and JH3-JH4 probes. PMN29 has at least 11 amino acid substitutions, which is an unusually large amount of somatic mutation for an IgM antibody. Previous analyses of BALB/c genomic libraries with VHX24 and VH441 probes make it unlikely that this VH family contains additional germ-line genes, but this possibility cannot be excluded. All three antibodies use the DQ52 and JH4 gene segments. The single VH and VL gene segments used to encode the anti-3-FL antibodies is in contrast to the multiple VH and VL segments used by antibodies against other carbohydrate Ag such as alpha 1-6 dextran and group A streptococcal carbohydrate. VH441 also encodes the VH regions of antibodies against galactan and levan (beta 2-6 fructosan). The similarities among VH segments of antibodies against 3-FL, levan, and galactan, and the striking differences in their CDR3 sequences, suggest that CDR3 plays an important role in the formation of the Ag binding site. The use of a single VH segment from the smallest VH gene family by antibodies against at least three different carbohydrate determinants is noteworthy. It raises the possibility that the amino acid sequence encoded by VH441 has some general structural features that make it particularly well adapted for binding to carbohydrate sequences." @default.
• W1676059852 created "2016-06-24" @default.
• W1676059852 creator A5003937757 @default.
• W1676059852 creator A5005872444 @default.
• W1676059852 creator A5028742878 @default.
• W1676059852 creator A5039463943 @default.
• W1676059852 creator A5050361931 @default.
• W1676059852 creator A5081952589 @default.
• W1676059852 creator A5084665624 @default.
• W1676059852 date "1988-02-15" @default.
• W1676059852 modified "2023-10-06" @default.
• W1676059852 title "Sequences of the VH and VL regions of murine monoclonal antibodies against 3-fucosyllactosamine." @default.
• W1676059852 doi "https://doi.org/10.4049/jimmunol.140.4.1212" @default.
• W1676059852 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2893825" @default.
• W1676059852 hasPublicationYear "1988" @default.
• W1676059852 type Work @default.
• W1676059852 sameAs 1676059852 @default.
• W1676059852 citedByCount "15" @default.
• W1676059852 countsByYear W16760598522013 @default.
• W1676059852 crossrefType "journal-article" @default.
• W1676059852 hasAuthorship W1676059852A5003937757 @default.
• W1676059852 hasAuthorship W1676059852A5005872444 @default.
• W1676059852 hasAuthorship W1676059852A5028742878 @default.
• W1676059852 hasAuthorship W1676059852A5039463943 @default.
• W1676059852 hasAuthorship W1676059852A5050361931 @default.
• W1676059852 hasAuthorship W1676059852A5081952589 @default.
• W1676059852 hasAuthorship W1676059852A5084665624 @default.
• W1676059852 hasBestOaLocation W16760598521 @default.
• W1676059852 hasConcept C104317684 @default.
• W1676059852 hasConcept C109825262 @default.
• W1676059852 hasConcept C141231307 @default.
• W1676059852 hasConcept C153911025 @default.
• W1676059852 hasConcept C159654299 @default.
• W1676059852 hasConcept C167625842 @default.
• W1676059852 hasConcept C515207424 @default.
• W1676059852 hasConcept C542903549 @default.
• W1676059852 hasConcept C54355233 @default.
• W1676059852 hasConcept C5911399 @default.
• W1676059852 hasConcept C86803240 @default.
• W1676059852 hasConceptScore W1676059852C104317684 @default.
• W1676059852 hasConceptScore W1676059852C109825262 @default.
• W1676059852 hasConceptScore W1676059852C141231307 @default.
• W1676059852 hasConceptScore W1676059852C153911025 @default.
• W1676059852 hasConceptScore W1676059852C159654299 @default.
• W1676059852 hasConceptScore W1676059852C167625842 @default.
• W1676059852 hasConceptScore W1676059852C515207424 @default.
• W1676059852 hasConceptScore W1676059852C542903549 @default.
• W1676059852 hasConceptScore W1676059852C54355233 @default.
• W1676059852 hasConceptScore W1676059852C5911399 @default.
• W1676059852 hasConceptScore W1676059852C86803240 @default.
• W1676059852 hasIssue "4" @default.
• W1676059852 hasLocation W16760598521 @default.
• W1676059852 hasLocation W16760598522 @default.
• W1676059852 hasOpenAccess W1676059852 @default.
• W1676059852 hasPrimaryLocation W16760598521 @default.
• W1676059852 hasRelatedWork W1968526004 @default.
• W1676059852 hasRelatedWork W2007559212 @default.
• W1676059852 hasRelatedWork W2013108791 @default.
• W1676059852 hasRelatedWork W2122540340 @default.
• W1676059852 hasRelatedWork W2334012009 @default.
• W1676059852 hasRelatedWork W2368460920 @default.
• W1676059852 hasRelatedWork W2378868006 @default.
• W1676059852 hasRelatedWork W2380360044 @default.
• W1676059852 hasRelatedWork W3129501535 @default.
• W1676059852 hasRelatedWork W4386091216 @default.
• W1676059852 hasVolume "140" @default.
• W1676059852 isParatext "false" @default.
• W1676059852 isRetracted "false" @default.
• W1676059852 magId "1676059852" @default.
• W1676059852 workType "article" @default.