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- W1676406229 abstract "By the use of a MatriGrid® we have established a three-dimensional high density cell culture. The MatriGrid® is a culture medium permeable, polymeric scaffold with 187 microcavities. In these cavities (300 μm diameter and 207 μm deep) the cells can growth three-dimensionally. For these experiments we measured the oxygen consumption of HepG2 cell cultures in order to optimize cultivation conditions. We measured and compared the oxygen consumption, growth rate and vitality under three different cultivation conditions: monolayer, three-dimensional static and three-dimensional actively perfused. The results show that the cells in a three-dimensional cell culture consume less oxygen as in a monolayer cell culture and that the actively perfused three-dimensional cell culture in the MatriGrid® has a similar growth rate and vitality as the monolayer culture." @default.
- W1676406229 created "2016-06-24" @default.
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- W1676406229 date "2013-04-22" @default.
- W1676406229 modified "2023-10-14" @default.
- W1676406229 title "Analysis and comparison of oxygen consumption of HepG2 cells in a monolayer and three‐dimensional high density cell culture by use of a matrigrid®" @default.
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- W1676406229 doi "https://doi.org/10.1002/bit.24912" @default.
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