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• W1676768139 abstract "A committed precursor of tetrapyrroles, δ -aminolevulinic acid (ALA), stimulates carotenoid accumulation when applied exogenously to cucumber cotyledons and to Euglena gracilis . The chapter shows that this stimulation is reversed by the chlorosis-inducing herbicide 3-amino-1,2,4-triazole (AT). It is impractical to generate the cucumber cotyledons in a completely aseptic manner, but the use of initially sterile solutions, glassware, and instruments significantly reduces variability and minimizes losses due to contamination. Cucumber seeds are generally coated with pink fungicide that is removed by washing in a sieve with cold tap water for about 1min, followed by surface sterilization in 5% commercial bleach for 5 min. Seeds, in 50-ml batches, are transferred to 100 ml of 2mM sodium phosphate buffer with or without AT in sterile 250 ml Erlenmeyer flasks. The addition of AT at this stage leads to much higher levels of the inhibition of pigment accumulation than simple preincubation alone." @default.
• W1676768139 created "2016-06-24" @default.
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• W1676768139 date "1993-01-01" @default.
• W1676768139 modified "2023-09-27" @default.
• W1676768139 title "[31] Effects of δ-aminolevulinic acid and 3-amino-1,2,4-triazole on carotenoid accumulation" @default.
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• W1676768139 doi "https://doi.org/10.1016/0076-6879(93)14078-w" @default.
• W1676768139 hasPublicationYear "1993" @default.
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