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• W1677986074 abstract "Abstract Background Transforming Growth Factor-β (TGF-β) regulates key biological processes during development and in adult tissues and has been implicated in many diseases. To study the biological functions of TGF-β, sensitive, specific, and convenient bioassays are necessary. Here we describe a new cell-based bioassay that fulfills these requirements. Results Embryonic fibroblasts from Tgfb1 -/- mice were stably transfected with a reporter plasmid consisting of TGF-β responsive Smad-binding elements coupled to a secreted alkaline phosphatase reporter gene (SBE-SEAP). Clone MFB-F11 showed more than 1000-fold induction after stimulation with 1 ng/ml TGF-β1, and detected as little as 1 pg/ml TGF-β1. MFB-F11 cells were highly induced by TGF-β1, TGF-β2 and TGF-β3, but did not show induction with related family members activin, nodal, BMP-2 and BMP-6 or with trophic factors bFGF and BDNF. MFB-F11 cells can detect and quantify TGF-β in biological samples without prior enrichment of TGF-βs, and can detect biologically activated TGF-β in a cell co-culture system. Conclusion MFB-F11 cells can be used to rapidly and specifically measure TGF-β with high sensitivity." @default.
• W1677986074 created "2016-06-24" @default.
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• W1677986074 date "2006-03-20" @default.
• W1677986074 modified "2023-10-12" @default.
• W1677986074 title "Highly sensitive and specific bioassay for measuring bioactive TGF-β" @default.
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• W1677986074 doi "https://doi.org/10.1186/1471-2121-7-15" @default.
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