Matches in SemOpenAlex for { <https://semopenalex.org/work/W1678804523> ?p ?o ?g. }
- W1678804523 endingPage "867" @default.
- W1678804523 startingPage "856" @default.
- W1678804523 abstract "J. Neurochem. (2012) 122, 856–867. Abstract Manganese (Mn) has been implicated in the impairment of the glutamate–glutamine cycling (GGC) by deregulation of Glu and glutamine (Gln) turnover in astrocytes. Here, we have examined possible mechanisms involved in the Mn(II)-mediated disruption of Glu turnover, including those related to protein degradation, such as the proteasomal and lysosomal machinery. Our study revealed that lysosome but not proteasomal inhibition is responsible for down-regulation of the Glu transporter after Mn(II) treatment. Because protein kinase C (PKC) activation leads to the down-regulation of Glu carriers, and Mn(II) increases PKC activity, we hypothesized that the PKC signaling contributes to the Mn(II)-mediated disruption of Glu turnover. Our results show that PKC activation causes a decrease in Glu uptake and that inhibition of PKC reverses Mn(II)-dependent down-regulation of Glu influx as well as glutamate transporter 1 (GLT1) and glutamate–aspartate transporter (GLAST) protein level. Co-immunoprecipitation studies show association of GLT1 with the PKCδ and PKCα isoforms and Mn(II)-induced specific increase in PKCδ-GLT1 interaction. In addition, astrocytes transfected with shRNA against PKCδ show decreased sensitivity to Mn(II) compared with those transfected with control shRNA or shRNA targeted against PKCα. Taken together, these findings demonstrate that PKCδ signaling is involved in the Mn(II)-induced deregulation of Glu turnover in astrocytes." @default.
- W1678804523 created "2016-06-24" @default.
- W1678804523 creator A5011456672 @default.
- W1678804523 creator A5026931772 @default.
- W1678804523 creator A5035503566 @default.
- W1678804523 date "2012-07-09" @default.
- W1678804523 modified "2023-10-03" @default.
- W1678804523 title "Mechanism of Mn(II)-mediated dysregulation of glutamine-glutamate cycle: focus on glutamate turnover" @default.
- W1678804523 cites W1507367785 @default.
- W1678804523 cites W1957958801 @default.
- W1678804523 cites W1965857385 @default.
- W1678804523 cites W1973154344 @default.
- W1678804523 cites W1977371894 @default.
- W1678804523 cites W1983685907 @default.
- W1678804523 cites W1984384644 @default.
- W1678804523 cites W2009139399 @default.
- W1678804523 cites W2010509965 @default.
- W1678804523 cites W2010857880 @default.
- W1678804523 cites W2012430517 @default.
- W1678804523 cites W2022957364 @default.
- W1678804523 cites W2034978650 @default.
- W1678804523 cites W2037351767 @default.
- W1678804523 cites W2039581146 @default.
- W1678804523 cites W2043286349 @default.
- W1678804523 cites W2053124649 @default.
- W1678804523 cites W2057737664 @default.
- W1678804523 cites W2058990053 @default.
- W1678804523 cites W2066181461 @default.
- W1678804523 cites W2066473958 @default.
- W1678804523 cites W2067087475 @default.
- W1678804523 cites W2068872653 @default.
- W1678804523 cites W2070255471 @default.
- W1678804523 cites W2070985058 @default.
- W1678804523 cites W2076463547 @default.
- W1678804523 cites W2076989525 @default.
- W1678804523 cites W2086731122 @default.
- W1678804523 cites W2088846224 @default.
- W1678804523 cites W2089745619 @default.
- W1678804523 cites W2092043203 @default.
- W1678804523 cites W2093414544 @default.
- W1678804523 cites W2094046759 @default.
- W1678804523 cites W2095266989 @default.
- W1678804523 cites W2126364360 @default.
- W1678804523 cites W2128744963 @default.
- W1678804523 cites W2134004958 @default.
- W1678804523 cites W2136147592 @default.
- W1678804523 cites W2138561233 @default.
- W1678804523 cites W2145666242 @default.
- W1678804523 cites W2145707016 @default.
- W1678804523 cites W2147944081 @default.
- W1678804523 cites W2160253297 @default.
- W1678804523 cites W2163107733 @default.
- W1678804523 cites W2167236182 @default.
- W1678804523 cites W228081133 @default.
- W1678804523 cites W4210979710 @default.
- W1678804523 cites W4232723971 @default.
- W1678804523 doi "https://doi.org/10.1111/j.1471-4159.2012.07835.x" @default.
- W1678804523 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3399925" @default.
- W1678804523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22708868" @default.
- W1678804523 hasPublicationYear "2012" @default.
- W1678804523 type Work @default.
- W1678804523 sameAs 1678804523 @default.
- W1678804523 citedByCount "29" @default.
- W1678804523 countsByYear W16788045232013 @default.
- W1678804523 countsByYear W16788045232014 @default.
- W1678804523 countsByYear W16788045232015 @default.
- W1678804523 countsByYear W16788045232017 @default.
- W1678804523 countsByYear W16788045232018 @default.
- W1678804523 countsByYear W16788045232019 @default.
- W1678804523 countsByYear W16788045232020 @default.
- W1678804523 countsByYear W16788045232021 @default.
- W1678804523 crossrefType "journal-article" @default.
- W1678804523 hasAuthorship W1678804523A5011456672 @default.
- W1678804523 hasAuthorship W1678804523A5026931772 @default.
- W1678804523 hasAuthorship W1678804523A5035503566 @default.
- W1678804523 hasBestOaLocation W16788045232 @default.
- W1678804523 hasConcept C104317684 @default.
- W1678804523 hasConcept C170493617 @default.
- W1678804523 hasConcept C185592680 @default.
- W1678804523 hasConcept C190232843 @default.
- W1678804523 hasConcept C195794163 @default.
- W1678804523 hasConcept C2779289688 @default.
- W1678804523 hasConcept C2779349466 @default.
- W1678804523 hasConcept C515207424 @default.
- W1678804523 hasConcept C55493867 @default.
- W1678804523 hasConcept C61174792 @default.
- W1678804523 hasConcept C62478195 @default.
- W1678804523 hasConcept C67705224 @default.
- W1678804523 hasConcept C86803240 @default.
- W1678804523 hasConcept C95444343 @default.
- W1678804523 hasConceptScore W1678804523C104317684 @default.
- W1678804523 hasConceptScore W1678804523C170493617 @default.
- W1678804523 hasConceptScore W1678804523C185592680 @default.
- W1678804523 hasConceptScore W1678804523C190232843 @default.
- W1678804523 hasConceptScore W1678804523C195794163 @default.
- W1678804523 hasConceptScore W1678804523C2779289688 @default.
- W1678804523 hasConceptScore W1678804523C2779349466 @default.
- W1678804523 hasConceptScore W1678804523C515207424 @default.