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• W1680326066 abstract "Namalwa and Raji cells, originally obtained from a Burkitt tumor biopsy, grow as continuous cell lines in vitro and contain the Epstein-Barr virus (EBV)-related nuclear antigen EBNA (B. M. Reedman and G. Klein, Int. J. Cancer 11 :499-520, 1973) and RNA homologous to at least 17 and 30% of the EBV genome, respectively (S. D. Hayward and E. Kieff, J. Virol. 18 :518-525, 1976; T. Orellana and E. Kieff, J. Virol. 22 :321-330, 1977). The polyribosomal and polyadenylated [poly(A)+] RNA fractions of Namalwa and Raji cells are enriched for a class of viral RNA homologous to 5 to 7% of EBV DNA (Hayward and Kieff, J. Virol. 18 :518-525, 1976; Orellana and Kieff, J. Virol. 22 :321-330, 1977). The objective of the experiments described in this communication was to determine the location within the map of the EBV genome (D. Given and E. Kieff, J. Virol. 28 :524-542, 1978) of the DNA which encodes the viral RNA in the poly(A)+ and non-polyadenylated [poly(A)−] RNA fractions of Namalwa cells. Hybridization of labeled DNA homologous to Namalwa poly(A)+ or poly(A)− RNA to blots containing Eco RI, Hsu I, or Hsu I/ Eco RI double-cut fragments of EBV (B95-8) or (W91) DNA indicated that these RNAs are encoded by DNA contained primarily in the Hsu I A/ Eco RI A and Hsu I B/ Eco RI A fragments and, to a lesser extent, in other fragments of the EBV genome. Hybridizations of Namalwa poly(A)+ and poly(A)− RNA in solution to denatured labeled Eco RI A or B fragments, Hsu I A, B, or D fragments, and Hsu I A/ Eco RI A or Bam I S fragments and of Raji polyribosomal poly(A)+ RNA to the Eco RI A fragment indicated that (i) Namalwa poly(A)+ RNA is encoded primarily by 6 × 10 5 daltons of a 2 × 10 6 -dalton segment of DNA, Bam I S, which is tandemly reiterated, approximately 10 times, in the Hsu I A/ Eco RI A fragment and is encoded to a lesser extent by DNA in the Hsu I B, Eco RI B, and Hsu I D fragments. Raji polyribosomal poly(A)+ RNA is encoded by a similar fraction of the Eco RI A fragment as that which encodes Namalwa poly(A)+ RNA. (ii) The fraction of the Bam I S fragment homologous to Namalwa poly(A)− RNA is similar to the fraction homologous to Namalwa poly(A)+ RNA. However, Namalwa poly(A)− RNA is homologous to a larger fraction of the DNA in the Hsu I B, Hsu I D, and Eco RI B fragments." @default.
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• W1680326066 date "1979-01-01" @default.
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• W1680326066 title "Epstein-Barr Virus-Specific RNA III. Mapping of DNA Encoding Viral RNA in Restringent Infection" @default.
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• W1680326066 doi "https://doi.org/10.1128/jvi.29.1.261-274.1979" @default.
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