FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1684170753> ?p ?o ?g. }

• W1684170753 endingPage "205" @default.
• W1684170753 startingPage "196" @default.
• W1684170753 abstract "Herpes simplex virus (HSV) encodes a ribonucleotide reductase consisting of two subunits (140 and 38 kilodaltons) whose genes map to coordinates 0.56 to 0.60 on the viral genome. Host cell lines containing the HpaI F fragment which includes the reductase subunit genes of HSV type 1 strain KOS (coordinates 0.535 to 0.620) were generated. Transfection of these cells with a plasmid containing the immediate-early ICP0 gene resulted in the expression of ICP6; interestingly, ICP4 plasmids failed to induce expression, indicating an unusual pattern of ICP6 regulation. One such cell line (D14) was used to isolate a mutant with the structural gene of lacZ inserted into the ICP6 gene such that the lacZ gene is read in frame with the N-terminal region of ICP6. This mutant generated a protein containing 434 amino acids (38%) of the N terminus of ICP6 fused to beta-galactosidase under control of the endogenous ICP6 promoter. Screening for virus recombinants was greatly facilitated by staining virus plaques with 5-bromo-4-chloro-3-indoyl-beta-D-galactoside (X-gal). Enzyme assays of infected BHK cells indicated that the mutant is incapable of inducing viral ribonucleotide reductase activity. Surprisingly, although plaque size was greatly reduced, mutant virus yield was reduced only four- to fivefold compared with that of the wild type grown in exponentially growing Vero cells. Mutant virus plaque size, yields, and ability to synthesize viral DNA were more severely compromised in serum-starved cells as compared with the wild type grown under the same condition. Although our evidence suggests that the HSV type 1 ribonucleotide reductase is not required for virus growth and DNA replication in dividing cells, it may be required for growth in nondividing cells." @default.
• W1684170753 created "2016-06-24" @default.
• W1684170753 creator A5025986669 @default.
• W1684170753 creator A5065763995 @default.
• W1684170753 date "1988-01-01" @default.
• W1684170753 modified "2023-10-16" @default.
• W1684170753 title "Herpes simplex virus type 1-induced ribonucleotide reductase activity is dispensable for virus growth and DNA synthesis: isolation and characterization of an ICP6 lacZ insertion mutant" @default.
• W1684170753 cites W123433535 @default.
• W1684170753 cites W1490731141 @default.
• W1684170753 cites W1504751521 @default.
• W1684170753 cites W1507725858 @default.
• W1684170753 cites W1513981021 @default.
• W1684170753 cites W1516191360 @default.
• W1684170753 cites W1554029096 @default.
• W1684170753 cites W1572756764 @default.
• W1684170753 cites W1580950619 @default.
• W1684170753 cites W1593604275 @default.
• W1684170753 cites W1595008575 @default.
• W1684170753 cites W1608801180 @default.
• W1684170753 cites W1613555203 @default.
• W1684170753 cites W1731704781 @default.
• W1684170753 cites W1774483545 @default.
• W1684170753 cites W1846625983 @default.
• W1684170753 cites W1849389588 @default.
• W1684170753 cites W1862850671 @default.
• W1684170753 cites W1891768184 @default.
• W1684170753 cites W1931233319 @default.
• W1684170753 cites W1947168737 @default.
• W1684170753 cites W1965372133 @default.
• W1684170753 cites W1985293411 @default.
• W1684170753 cites W1993491383 @default.
• W1684170753 cites W1995556647 @default.
• W1684170753 cites W1995951956 @default.
• W1684170753 cites W1996498777 @default.
• W1684170753 cites W1999273121 @default.
• W1684170753 cites W2014669590 @default.
• W1684170753 cites W2018289835 @default.
• W1684170753 cites W2035091776 @default.
• W1684170753 cites W2038634220 @default.
• W1684170753 cites W2042370689 @default.
• W1684170753 cites W2048246686 @default.
• W1684170753 cites W2048575806 @default.
• W1684170753 cites W2050744150 @default.
• W1684170753 cites W2053681932 @default.
• W1684170753 cites W2063450941 @default.
• W1684170753 cites W2066827421 @default.
• W1684170753 cites W2078302353 @default.
• W1684170753 cites W2088266710 @default.
• W1684170753 cites W2096211774 @default.
• W1684170753 cites W2100034740 @default.
• W1684170753 cites W2100837269 @default.
• W1684170753 cites W2110360181 @default.
• W1684170753 cites W2114240169 @default.
• W1684170753 cites W2115992829 @default.
• W1684170753 cites W2118929543 @default.
• W1684170753 cites W2124694752 @default.
• W1684170753 cites W2140938030 @default.
• W1684170753 cites W2157911990 @default.
• W1684170753 cites W2165554716 @default.
• W1684170753 cites W2167414261 @default.
• W1684170753 cites W2178778111 @default.
• W1684170753 cites W2254058835 @default.
• W1684170753 doi "https://doi.org/10.1128/jvi.62.1.196-205.1988" @default.
• W1684170753 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/250519" @default.
• W1684170753 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2824847" @default.
• W1684170753 hasPublicationYear "1988" @default.
• W1684170753 type Work @default.
• W1684170753 sameAs 1684170753 @default.
• W1684170753 citedByCount "368" @default.
• W1684170753 countsByYear W16841707532012 @default.
• W1684170753 countsByYear W16841707532013 @default.
• W1684170753 countsByYear W16841707532014 @default.
• W1684170753 countsByYear W16841707532015 @default.
• W1684170753 countsByYear W16841707532016 @default.
• W1684170753 countsByYear W16841707532017 @default.
• W1684170753 countsByYear W16841707532018 @default.
• W1684170753 countsByYear W16841707532019 @default.
• W1684170753 countsByYear W16841707532020 @default.
• W1684170753 countsByYear W16841707532021 @default.
• W1684170753 countsByYear W16841707532022 @default.
• W1684170753 countsByYear W16841707532023 @default.
• W1684170753 crossrefType "journal-article" @default.
• W1684170753 hasAuthorship W1684170753A5025986669 @default.
• W1684170753 hasAuthorship W1684170753A5065763995 @default.
• W1684170753 hasBestOaLocation W16841707531 @default.
• W1684170753 hasConcept C104292427 @default.
• W1684170753 hasConcept C104317684 @default.
• W1684170753 hasConcept C143065580 @default.
• W1684170753 hasConcept C153911025 @default.
• W1684170753 hasConcept C159047783 @default.
• W1684170753 hasConcept C22744801 @default.
• W1684170753 hasConcept C2522874641 @default.
• W1684170753 hasConcept C2781196997 @default.
• W1684170753 hasConcept C4710235 @default.
• W1684170753 hasConcept C54009773 @default.
• W1684170753 hasConcept C55493867 @default.
• W1684170753 hasConcept C86803240 @default.
• W1684170753 hasConcept C89138240 @default.

• next