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• W1688350895 abstract "[ <TEX>$DeniLite^{TM}$</TEX> ] laccase immobilized Pt electrode was used for amperometric detection of some catechol derivatives and o-aminophenol (OAP) derivative by means of substrate recycling. In case of catechol derivatives, the obtained sensitivities are 85, 79 and <TEX>$57 nA/{mu}M$</TEX> with linear ranges of <TEX>$0.6~30,;0.6~30;and; 1~25 {mu}M$</TEX> and detection limits (S/N=3) of 0.2, 0.2 and <TEX>$0.3{mu}M$</TEX> for 3,4-dihydroxycinnaminic acid (3,4-DHCA), 3,4-dihydroxybenzoic acid (3,4-DHBA) and 3,4-dihydroxyphenylacetic acid (3,4-DHPAA), respectively. In case of OAP derivative, the obtained sensitivity is <TEX>$237 nA/{mu}M$</TEX> with linear range of <TEX>$0.2~15{mu}M$</TEX> and detection limit of 70 nM for 2-amino-4-chlorophenol (2-A-4-CP). The response time <TEX>$(t_{90%})$</TEX> is about 2 seconds for each substrate and the long-term stability is around 40-50days for catechol derivatives and 30 days for 2-A-4-CP with retaining <TEX>$80%$</TEX> of initial activity. The optimal pHs of the sensor for these substrates are in the range of 4.5-5.0, which indicates that stability of the enzymatically oxidized product plays a very important role in substrate recycling. The different sensitivity of the sensor for each substrate can be explained by the electronic effect of the sugstituent on the enzymatically oxidized form." @default.
• W1688350895 created "2016-06-24" @default.
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• W1688350895 date "2004-05-01" @default.
• W1688350895 modified "2023-09-26" @default.
• W1688350895 title "Amperometric Detection of Some Catechol Derivatives and o-aminophenol Derivative with Laccase Immobilized Electrode: Effect of Substrate Structure" @default.
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• W1688350895 doi "https://doi.org/10.5229/jkes.2004.7.2.083" @default.
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