Matches in SemOpenAlex for { <https://semopenalex.org/work/W169268049> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W169268049 endingPage "16" @default.
- W169268049 startingPage "1306" @default.
- W169268049 abstract "To identify and synthesize the polymorphonuclear leukocyte chemoattractant(s) released from alkali-degraded corneas.Corneas were degraded in 1.0 N NaOH, neutralized, ultrafiltered, and dialyzed. The final active ultrafiltrate was subjected to high-performance liquid chromatography on a Protein PAK I-60 column. The most active fractions were further separated on a mu-Bondapak-C18 and I-60 column in sequence.Fraction 38 from the final I-60 column associated with a 210-nm absorption peak and elicited a polarization and chemotactic response from polymorphonuclear leukocytes. The loss of polarization activity in fraction 38 after exposure to prolidase suggests that this peptide contains a Pro-X (X = amino acid) peptide bond. The amino acid composition of fraction 38 was 35% glycine and 53% proline. Peptide sequence analysis was unable to establish a primary sequence even though Picotag analysis showed the presence of large amounts of the two amino acids. Mass spectrometry revealed only two molecular species of 312 MWt and 284 MWt. Tripeptides were synthesized using all possible amino acid permutations of 2 Pro and 1 Gly and tested in the polarization and chemotactic assays. These techniques demonstrated that n-acetyl-Pro-Gly-Pro, and to a lesser degree n-methyl-Pro-Gly-Pro, were the only synthetic tripeptides with activity similar to the purified chemoattractant.The data show that the chemotactic peptides, purified from alkali-degraded whole cornea and confirmed with identical synthetic tripeptides, are N-acetyl-Pro-Gly-Pro and N-methyl-Pro-Gly-Pro. Although a number of proteins contain the Pro-Gly-Pro sequence, large amounts of collagen in the cornea suggest this as a major source. The small size and hydrophilic nature of these chemoattractants are predictive of a high degree of diffusibility. These chemoattractants are likely to play a major role in the early neutrophil response after an alkali injury." @default.
- W169268049 created "2016-06-24" @default.
- W169268049 creator A5025174065 @default.
- W169268049 creator A5040592884 @default.
- W169268049 creator A5068753140 @default.
- W169268049 creator A5070389365 @default.
- W169268049 date "1995-06-01" @default.
- W169268049 modified "2023-09-23" @default.
- W169268049 title "Identification and synthesis of chemotactic tripeptides from alkali-degraded whole cornea. A study of N-acetyl-proline-glycine-proline and N-methyl-proline-glycine-proline." @default.
- W169268049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7775108" @default.
- W169268049 hasPublicationYear "1995" @default.
- W169268049 type Work @default.
- W169268049 sameAs 169268049 @default.
- W169268049 citedByCount "31" @default.
- W169268049 countsByYear W1692680492012 @default.
- W169268049 countsByYear W1692680492014 @default.
- W169268049 countsByYear W1692680492015 @default.
- W169268049 countsByYear W1692680492016 @default.
- W169268049 countsByYear W1692680492017 @default.
- W169268049 countsByYear W1692680492018 @default.
- W169268049 countsByYear W1692680492020 @default.
- W169268049 crossrefType "journal-article" @default.
- W169268049 hasAuthorship W169268049A5025174065 @default.
- W169268049 hasAuthorship W169268049A5040592884 @default.
- W169268049 hasAuthorship W169268049A5068753140 @default.
- W169268049 hasAuthorship W169268049A5070389365 @default.
- W169268049 hasConcept C104317684 @default.
- W169268049 hasConcept C167392928 @default.
- W169268049 hasConcept C167625842 @default.
- W169268049 hasConcept C185592680 @default.
- W169268049 hasConcept C2777756961 @default.
- W169268049 hasConcept C2779281246 @default.
- W169268049 hasConcept C2779815552 @default.
- W169268049 hasConcept C43617362 @default.
- W169268049 hasConcept C515207424 @default.
- W169268049 hasConcept C55493867 @default.
- W169268049 hasConceptScore W169268049C104317684 @default.
- W169268049 hasConceptScore W169268049C167392928 @default.
- W169268049 hasConceptScore W169268049C167625842 @default.
- W169268049 hasConceptScore W169268049C185592680 @default.
- W169268049 hasConceptScore W169268049C2777756961 @default.
- W169268049 hasConceptScore W169268049C2779281246 @default.
- W169268049 hasConceptScore W169268049C2779815552 @default.
- W169268049 hasConceptScore W169268049C43617362 @default.
- W169268049 hasConceptScore W169268049C515207424 @default.
- W169268049 hasConceptScore W169268049C55493867 @default.
- W169268049 hasIssue "7" @default.
- W169268049 hasLocation W1692680491 @default.
- W169268049 hasOpenAccess W169268049 @default.
- W169268049 hasPrimaryLocation W1692680491 @default.
- W169268049 hasRelatedWork W169268049 @default.
- W169268049 hasRelatedWork W1830352000 @default.
- W169268049 hasRelatedWork W1975319264 @default.
- W169268049 hasRelatedWork W203216892 @default.
- W169268049 hasRelatedWork W2048831462 @default.
- W169268049 hasRelatedWork W2080498701 @default.
- W169268049 hasRelatedWork W2082505301 @default.
- W169268049 hasRelatedWork W2108725809 @default.
- W169268049 hasRelatedWork W2248063041 @default.
- W169268049 hasRelatedWork W2426906498 @default.
- W169268049 hasVolume "36" @default.
- W169268049 isParatext "false" @default.
- W169268049 isRetracted "false" @default.
- W169268049 magId "169268049" @default.
- W169268049 workType "article" @default.