FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W169350350> ?p ?o ?g. }

• W169350350 abstract "Cancer is one of the leading causes of death for people in the United States. Despite the numerous cancer treatments available, many of these chemotherapy treatments cause negative side effects such as hair and weight loss. With this in mind, in the past 15 years, researchers have discovered a mechanism known as RNA interference, which can be used to control certain genes and their activity. This mechanism is based on the function of siRNA molecules, which can be inserted into cells. These siRNA molecules can be manipulated to target and eliminate specific cancer cells. However, these siRNA molecules are normally associated with inconvenient problems such as instability and a short half-life. These siRNA molecules also face an uphill task of entering these targets cells. In most cases, cellular entryways are highly selective based on the size and type of cargo. With this in mind, researchers have discovered that gold nanoparticles (AuNPs) can be a suitable transport vehicle for siRNA molecules into targeted cells due to their easy synthesis, easy modification for specific pathways, and high biocompatibility with living tissue. Studies have shown that AuNPs can increase the half-life of siRNA molecules by six fold. Before assembling these gold nanoparticles with siRNA, they must be coated with a reagent of branched polyethyleneimine (C12-PEI) chains in order to increase the stability of the gold nanoparticles, thus producing newly-formed AuPEI conjugates. Subsequently, the PEI will bind with siRNA based on their electrostatic interaction, forming a well-dispersed nanoparticle with uniform size and narrow size distribution. In Fall 2011, the Huang research group encountered several problems with the synthesis of these AuPEI conjugates. These experiments revealed data suggesting that aggregation occurred at certain concentrations of PEI. Experimental data also suggested that aggregation had occurred when loading these AuPEI conjugates with tRNA. With these problems in mind, the Spring 2012 semester of research has been geared towards determining the optimal PEI concentration for the synthesis of AuPEI conjugates. More specifically, the research effort was geared towards exploring how many PEI molecules are bound to each gold nanoparticle. The Huang research group designed a C12PEI solution incorporated with fluorescein isothiocyanate (FITC) in order to gain a closer look into the synthesis of these AuPEI-FITC conjugates. By monitoring the fluorescence of these AuPEI-FITC conjugates, the results could provide information about optimal synthesis conditions. The Huang research group has conducted numerous experiments to study the effects of incubation time, temperature, and DTT (dithiothreitol) concentration on the amount of PEI-FITC on each gold nanoparticle. Based on the experimental results and its statistical analysis, the Huang research group has successfully determined that an estimated 1,000 PEI molecules are bound to each gold nanoparticle. Upon determining PEI density, the goal of the Summer 2012 semester was to optimize the AuPEItRNA conjugate assembly system. Based on extensive experimental data and analysis, the optimized conditions for assembly have been established for future research efforts. v" @default.
• W169350350 created "2016-06-24" @default.
• W169350350 creator A5062738864 @default.
• W169350350 date "2013-01-01" @default.
• W169350350 modified "2023-09-27" @default.
• W169350350 title "The Characterization of AuNP-PEI Conjugates for siRNA Delivery to Cancer Cells”" @default.
• W169350350 cites W1977125458 @default.
• W169350350 cites W1990377587 @default.
• W169350350 cites W2035544537 @default.
• W169350350 cites W2040640949 @default.
• W169350350 cites W2064614508 @default.
• W169350350 cites W2064848737 @default.
• W169350350 cites W2073658360 @default.
• W169350350 cites W2115210634 @default.
• W169350350 hasPublicationYear "2013" @default.
• W169350350 type Work @default.
• W169350350 sameAs 169350350 @default.
• W169350350 citedByCount "0" @default.
• W169350350 crossrefType "journal-article" @default.
• W169350350 hasAuthorship W169350350A5062738864 @default.
• W169350350 hasConcept C104317684 @default.
• W169350350 hasConcept C104819515 @default.
• W169350350 hasConcept C121608353 @default.
• W169350350 hasConcept C12554922 @default.
• W169350350 hasConcept C134306372 @default.
• W169350350 hasConcept C155672457 @default.
• W169350350 hasConcept C161624437 @default.
• W169350350 hasConcept C166703698 @default.
• W169350350 hasConcept C171250308 @default.
• W169350350 hasConcept C178790620 @default.
• W169350350 hasConcept C185592680 @default.
• W169350350 hasConcept C192562407 @default.
• W169350350 hasConcept C197336794 @default.
• W169350350 hasConcept C22615655 @default.
• W169350350 hasConcept C2777230088 @default.
• W169350350 hasConcept C33923547 @default.
• W169350350 hasConcept C54355233 @default.
• W169350350 hasConcept C55493867 @default.
• W169350350 hasConcept C67705224 @default.
• W169350350 hasConcept C86803240 @default.
• W169350350 hasConcept C95444343 @default.
• W169350350 hasConcept C96232424 @default.
• W169350350 hasConceptScore W169350350C104317684 @default.
• W169350350 hasConceptScore W169350350C104819515 @default.
• W169350350 hasConceptScore W169350350C121608353 @default.
• W169350350 hasConceptScore W169350350C12554922 @default.
• W169350350 hasConceptScore W169350350C134306372 @default.
• W169350350 hasConceptScore W169350350C155672457 @default.
• W169350350 hasConceptScore W169350350C161624437 @default.
• W169350350 hasConceptScore W169350350C166703698 @default.
• W169350350 hasConceptScore W169350350C171250308 @default.
• W169350350 hasConceptScore W169350350C178790620 @default.
• W169350350 hasConceptScore W169350350C185592680 @default.
• W169350350 hasConceptScore W169350350C192562407 @default.
• W169350350 hasConceptScore W169350350C197336794 @default.
• W169350350 hasConceptScore W169350350C22615655 @default.
• W169350350 hasConceptScore W169350350C2777230088 @default.
• W169350350 hasConceptScore W169350350C33923547 @default.
• W169350350 hasConceptScore W169350350C54355233 @default.
• W169350350 hasConceptScore W169350350C55493867 @default.
• W169350350 hasConceptScore W169350350C67705224 @default.
• W169350350 hasConceptScore W169350350C86803240 @default.
• W169350350 hasConceptScore W169350350C95444343 @default.
• W169350350 hasConceptScore W169350350C96232424 @default.
• W169350350 hasLocation W1693503501 @default.
• W169350350 hasOpenAccess W169350350 @default.
• W169350350 hasPrimaryLocation W1693503501 @default.
• W169350350 hasRelatedWork W1969313893 @default.
• W169350350 hasRelatedWork W1979322667 @default.
• W169350350 hasRelatedWork W1999550521 @default.
• W169350350 hasRelatedWork W2029996023 @default.
• W169350350 hasRelatedWork W2033625903 @default.
• W169350350 hasRelatedWork W2062495233 @default.
• W169350350 hasRelatedWork W2103029026 @default.
• W169350350 hasRelatedWork W2147364460 @default.
• W169350350 hasRelatedWork W2285781213 @default.
• W169350350 hasRelatedWork W2387285990 @default.
• W169350350 hasRelatedWork W2389366201 @default.
• W169350350 hasRelatedWork W2520301820 @default.
• W169350350 hasRelatedWork W2547173293 @default.
• W169350350 hasRelatedWork W2555689074 @default.
• W169350350 hasRelatedWork W2581524809 @default.
• W169350350 hasRelatedWork W2922283067 @default.
• W169350350 hasRelatedWork W2945039848 @default.
• W169350350 hasRelatedWork W30244953 @default.
• W169350350 hasRelatedWork W3112558789 @default.
• W169350350 hasRelatedWork W3155831444 @default.
• W169350350 isParatext "false" @default.
• W169350350 isRetracted "false" @default.
• W169350350 magId "169350350" @default.
• W169350350 workType "article" @default.