Matches in SemOpenAlex for { <https://semopenalex.org/work/W1693545577> ?p ?o ?g. }
- W1693545577 endingPage "4128" @default.
- W1693545577 startingPage "4114" @default.
- W1693545577 abstract "cDNA cloning of ecdysone receptor (EcR) and ultraspiracle (USP) of the coleopteran Colorado potato beetle Leptinotarsa decemlineata (LdEcR and LdUSP) was conducted. Amino‐acid sequences of the proteins deduced from cDNA sequences showed striking homology to those of other insects, especially the coleopteran yellow mealworm Tenebrio molitor . Northern hybridization analysis showed a 12.4‐kb message for the LdEcR A‐isoform, a 10.5‐kb message for the LdEcR B1‐isoform and a 5.7‐kb message for the LdUSP, in fat body, gut, integument, testis and ovaries. In developmental profile studies, expression of both the LdEcR and LdUSP transcript in integument changed dramatically. In gel mobility shift assays, in vitro translated LdEcR alone bound weakly to the pal1 ecdysone response element, although LdUSP alone did not, and this binding was dramatically enhanced by the addition of LdUSP. LdEcR/LdUSP complex also showed significant binding to an ecdysone agonist, ponasterone A ( K D = 2.8 n m ), while LdEcR alone showed only weak binding ( K D = 73.4 n m ), and LdUSP alone did not show any binding. The receptor‐binding affinity of various ecdysone agonists to LdEcR/LdUSP was not correlated to their larvicidal activity to L. decemlineata . From these results, it was suggested that multiple factors including the receptor binding affinity are related to the determination of the larvicidal activity of nonsteroidal ecdysone agonists in L. decemlineata ." @default.
- W1693545577 created "2016-06-24" @default.
- W1693545577 creator A5004972579 @default.
- W1693545577 creator A5020225564 @default.
- W1693545577 creator A5047693423 @default.
- W1693545577 creator A5066515722 @default.
- W1693545577 creator A5085001876 @default.
- W1693545577 date "2005-08-01" @default.
- W1693545577 modified "2023-10-18" @default.
- W1693545577 title "Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata" @default.
- W1693545577 cites W101761358 @default.
- W1693545577 cites W1522465402 @default.
- W1693545577 cites W1527837601 @default.
- W1693545577 cites W1569219515 @default.
- W1693545577 cites W1597939374 @default.
- W1693545577 cites W1881800315 @default.
- W1693545577 cites W1951209055 @default.
- W1693545577 cites W1964069111 @default.
- W1693545577 cites W1966452746 @default.
- W1693545577 cites W1966843280 @default.
- W1693545577 cites W1967750144 @default.
- W1693545577 cites W1968369737 @default.
- W1693545577 cites W1969069589 @default.
- W1693545577 cites W1970306476 @default.
- W1693545577 cites W1970357896 @default.
- W1693545577 cites W1973770403 @default.
- W1693545577 cites W1981939829 @default.
- W1693545577 cites W1983827669 @default.
- W1693545577 cites W1989296505 @default.
- W1693545577 cites W1993521976 @default.
- W1693545577 cites W1996237491 @default.
- W1693545577 cites W1996291500 @default.
- W1693545577 cites W1996893888 @default.
- W1693545577 cites W1997375606 @default.
- W1693545577 cites W2000517171 @default.
- W1693545577 cites W2000518052 @default.
- W1693545577 cites W2001752693 @default.
- W1693545577 cites W2003491070 @default.
- W1693545577 cites W2010537142 @default.
- W1693545577 cites W2011273002 @default.
- W1693545577 cites W2014631767 @default.
- W1693545577 cites W2016023815 @default.
- W1693545577 cites W2019125169 @default.
- W1693545577 cites W2023907428 @default.
- W1693545577 cites W2029994619 @default.
- W1693545577 cites W2031168482 @default.
- W1693545577 cites W2031925654 @default.
- W1693545577 cites W2034121682 @default.
- W1693545577 cites W2034975889 @default.
- W1693545577 cites W2042348276 @default.
- W1693545577 cites W2043274454 @default.
- W1693545577 cites W2044633482 @default.
- W1693545577 cites W2045299880 @default.
- W1693545577 cites W2046903905 @default.
- W1693545577 cites W2050839185 @default.
- W1693545577 cites W2056139391 @default.
- W1693545577 cites W2057708838 @default.
- W1693545577 cites W2058841426 @default.
- W1693545577 cites W2059291229 @default.
- W1693545577 cites W2062410683 @default.
- W1693545577 cites W2066310581 @default.
- W1693545577 cites W2075808105 @default.
- W1693545577 cites W2078606549 @default.
- W1693545577 cites W2083457602 @default.
- W1693545577 cites W2087517344 @default.
- W1693545577 cites W2091442347 @default.
- W1693545577 cites W2092576954 @default.
- W1693545577 cites W2093090457 @default.
- W1693545577 cites W2097978662 @default.
- W1693545577 cites W2102639583 @default.
- W1693545577 cites W2115296444 @default.
- W1693545577 cites W2136267625 @default.
- W1693545577 cites W2141657351 @default.
- W1693545577 cites W2149199637 @default.
- W1693545577 cites W2149662691 @default.
- W1693545577 cites W2152040532 @default.
- W1693545577 cites W2152414401 @default.
- W1693545577 cites W2165114488 @default.
- W1693545577 cites W4234609843 @default.
- W1693545577 doi "https://doi.org/10.1111/j.1742-4658.2005.04823.x" @default.
- W1693545577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16098194" @default.
- W1693545577 hasPublicationYear "2005" @default.
- W1693545577 type Work @default.
- W1693545577 sameAs 1693545577 @default.
- W1693545577 citedByCount "77" @default.
- W1693545577 countsByYear W16935455772012 @default.
- W1693545577 countsByYear W16935455772013 @default.
- W1693545577 countsByYear W16935455772014 @default.
- W1693545577 countsByYear W16935455772015 @default.
- W1693545577 countsByYear W16935455772016 @default.
- W1693545577 countsByYear W16935455772017 @default.
- W1693545577 countsByYear W16935455772018 @default.
- W1693545577 countsByYear W16935455772019 @default.
- W1693545577 countsByYear W16935455772020 @default.
- W1693545577 crossrefType "journal-article" @default.
- W1693545577 hasAuthorship W1693545577A5004972579 @default.
- W1693545577 hasAuthorship W1693545577A5020225564 @default.
- W1693545577 hasAuthorship W1693545577A5047693423 @default.