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- W1694040553 abstract "Summary The σ 54 factor is highly conserved in a large number of bacterial species. From the complete genome sequence of Rhodobacter sphaeroides , it was possible to identify four different sequences encoding potentially functional σ 54 factors. In this work, we provide evidence that one of these copies ( rpoN 2) is specifically required to express the flagellar genes in this bacterium. A mutant strain carrying a lesion in the rpoN2 gene was unable to swim even though the RpoN1 and RpoN3 proteins were present in the cytoplasm. The possibility that the different copies of the σ 54 factor might be specific for the transcription of a particular subset of σ 54 promoters was reinforced by the fact that a mutant strain carrying a lesion in rpoN1 showed a severe growth defect in nitrogen‐free culture medium, even though the rpoN2 and rpoN4 genes were actively transcribed from a plasmid or from the chromosome. Different mech‐anisms that might be responsible for this specificity are discussed." @default.
- W1694040553 created "2016-06-24" @default.
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- W1694040553 date "2002-10-01" @default.
- W1694040553 modified "2023-10-18" @default.
- W1694040553 title "The four different σ54 factors of Rhodobacter sphaeroides are not functionally interchangeable" @default.
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- W1694040553 doi "https://doi.org/10.1046/j.1365-2958.2002.03158.x" @default.
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