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- W1694920334 abstract "Ligand–protein interactions (LPIs) are fundamental biological processes that manipulate a variety of cellular events. While multiple LPIs can occur concurrently or concertedly at a cellular interface, techniques which are able to simultaneously probe these diverse interactions remain challenging. Here, to the best of our knowledge, the first fluorogenic composite material (FCM) is developed that can probe diverse LPIs at a single biomimetic interface. We determined that two glycoligands coupled with fluorescence dyes with different emission colors can coassemble to a graphene oxide platform, producing an integrated FCM with a quenched fluorescence. The fluorescence of each “glycodye” is uniquely elicited upon interaction with a pairing protein (lectin) that selectively recognizes the glycoligand. Importantly, the dual emission of the FCM can be produced, on a single excitation, while both proteins exist, providing a concise means for the simultaneous probing of diverse LPIs." @default.
- W1694920334 created "2016-06-24" @default.
- W1694920334 creator A5021324401 @default.
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- W1694920334 date "2015-05-04" @default.
- W1694920334 modified "2023-10-16" @default.
- W1694920334 title "Simultaneous Detection of Diverse Glycoligand-Receptor Recognitions Using a Single-Excitation, Dual-Emission Graphene Composite" @default.
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- W1694920334 doi "https://doi.org/10.1002/adfm.201500448" @default.
- W1694920334 hasPublicationYear "2015" @default.
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