Matches in SemOpenAlex for { <https://semopenalex.org/work/W16951500> ?p ?o ?g. }
- W16951500 endingPage "149" @default.
- W16951500 startingPage "141" @default.
- W16951500 abstract "Gene-activated matrix has wide potential utilization in tissue engineering. It may genetically modify cells with plasmid DNA encoding therapeutic genes and allow sustained expression and release of the proteins to surrounding tissues. In this study, we assessed the feasibility of the local gene release from human fibronectin (HFN) substrate and the efficacy of local release of stromal cell-derived factor-1 (SDF-1) gene on c-kit + cell homing. Cationic polymer polyethylenimine (25kDa PEI) was used as non-viral DNA vector. Gene-activated HFN (GAH) was prepared by mixing PEI/DNA complexes with HFN substrate. The DNA retardation, the complex size, and the DNA release speed from the GAH were studied. The in vitro transfection was optimized by luciferase expression and cell viability assay in the COS7 cell line. Localized gene expression in COS7 cells cultured on the GAH was assessed by LacZ and GFP-N3-SDF-1 marker genes. C-kit + cell homing was investigated in response to the local in vitro SDF-1 expression from rat mesenchymal stem cells (RMSCs) cultured on GAH. Results showed GAH allows long time-sustained DNA release, localized gene delivery, and high transfection efficiency. Local SDF-1 expression with GAH is a promising method to induce targetable stem cell homing." @default.
- W16951500 created "2016-06-24" @default.
- W16951500 creator A5004218295 @default.
- W16951500 creator A5005551453 @default.
- W16951500 creator A5010607811 @default.
- W16951500 creator A5020839405 @default.
- W16951500 creator A5024268530 @default.
- W16951500 creator A5032945918 @default.
- W16951500 creator A5042241049 @default.
- W16951500 creator A5045629455 @default.
- W16951500 creator A5047480214 @default.
- W16951500 creator A5055249856 @default.
- W16951500 creator A5068582168 @default.
- W16951500 creator A5084610792 @default.
- W16951500 date "2009-03-01" @default.
- W16951500 modified "2023-09-27" @default.
- W16951500 title "Localized and Sustained SDF-1 Gene Release Mediated by Fibronectin Films: A Potential Method for Recruiting Stem Cells" @default.
- W16951500 cites W1536781994 @default.
- W16951500 cites W1557673622 @default.
- W16951500 cites W1969095342 @default.
- W16951500 cites W1987568997 @default.
- W16951500 cites W1994344017 @default.
- W16951500 cites W2006967267 @default.
- W16951500 cites W2010655741 @default.
- W16951500 cites W2014771201 @default.
- W16951500 cites W2026615220 @default.
- W16951500 cites W2029231027 @default.
- W16951500 cites W2039163665 @default.
- W16951500 cites W2059444094 @default.
- W16951500 cites W2071715590 @default.
- W16951500 cites W2098211086 @default.
- W16951500 cites W2107111991 @default.
- W16951500 cites W2114918609 @default.
- W16951500 cites W2117412164 @default.
- W16951500 cites W2117752891 @default.
- W16951500 cites W2133496200 @default.
- W16951500 cites W2142226770 @default.
- W16951500 cites W2148164592 @default.
- W16951500 doi "https://doi.org/10.1177/039139880903200304" @default.
- W16951500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19440989" @default.
- W16951500 hasPublicationYear "2009" @default.
- W16951500 type Work @default.
- W16951500 sameAs 16951500 @default.
- W16951500 citedByCount "19" @default.
- W16951500 countsByYear W169515002012 @default.
- W16951500 countsByYear W169515002013 @default.
- W16951500 countsByYear W169515002014 @default.
- W16951500 countsByYear W169515002017 @default.
- W16951500 countsByYear W169515002023 @default.
- W16951500 crossrefType "journal-article" @default.
- W16951500 hasAuthorship W16951500A5004218295 @default.
- W16951500 hasAuthorship W16951500A5005551453 @default.
- W16951500 hasAuthorship W16951500A5010607811 @default.
- W16951500 hasAuthorship W16951500A5020839405 @default.
- W16951500 hasAuthorship W16951500A5024268530 @default.
- W16951500 hasAuthorship W16951500A5032945918 @default.
- W16951500 hasAuthorship W16951500A5042241049 @default.
- W16951500 hasAuthorship W16951500A5045629455 @default.
- W16951500 hasAuthorship W16951500A5047480214 @default.
- W16951500 hasAuthorship W16951500A5055249856 @default.
- W16951500 hasAuthorship W16951500A5068582168 @default.
- W16951500 hasAuthorship W16951500A5084610792 @default.
- W16951500 hasConcept C104317684 @default.
- W16951500 hasConcept C135983454 @default.
- W16951500 hasConcept C150194340 @default.
- W16951500 hasConcept C153911025 @default.
- W16951500 hasConcept C16930146 @default.
- W16951500 hasConcept C185592680 @default.
- W16951500 hasConcept C189165786 @default.
- W16951500 hasConcept C198826908 @default.
- W16951500 hasConcept C28328180 @default.
- W16951500 hasConcept C502942594 @default.
- W16951500 hasConcept C54009773 @default.
- W16951500 hasConcept C55493867 @default.
- W16951500 hasConcept C86492073 @default.
- W16951500 hasConcept C86803240 @default.
- W16951500 hasConcept C95444343 @default.
- W16951500 hasConceptScore W16951500C104317684 @default.
- W16951500 hasConceptScore W16951500C135983454 @default.
- W16951500 hasConceptScore W16951500C150194340 @default.
- W16951500 hasConceptScore W16951500C153911025 @default.
- W16951500 hasConceptScore W16951500C16930146 @default.
- W16951500 hasConceptScore W16951500C185592680 @default.
- W16951500 hasConceptScore W16951500C189165786 @default.
- W16951500 hasConceptScore W16951500C198826908 @default.
- W16951500 hasConceptScore W16951500C28328180 @default.
- W16951500 hasConceptScore W16951500C502942594 @default.
- W16951500 hasConceptScore W16951500C54009773 @default.
- W16951500 hasConceptScore W16951500C55493867 @default.
- W16951500 hasConceptScore W16951500C86492073 @default.
- W16951500 hasConceptScore W16951500C86803240 @default.
- W16951500 hasConceptScore W16951500C95444343 @default.
- W16951500 hasIssue "3" @default.
- W16951500 hasLocation W169515001 @default.
- W16951500 hasLocation W169515002 @default.
- W16951500 hasOpenAccess W16951500 @default.
- W16951500 hasPrimaryLocation W169515001 @default.
- W16951500 hasRelatedWork W144014998 @default.