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- W1695661112 abstract "Protochlorophyllide holochrome (PCH) is the protochlorophyllide–protein complex, which is photo-reduced to chlorophyllide holochrome. It has been obtained primarily from dark-grown bean leaves. This chapter describes an experiment, which was conducted to study isolation and purification procedure o provide reasonable amounts of PCH. The absorption spectra of solutions were measured before and after a saturating red irradiation in 1 cm cells at 10 ° C with a Cary Model 14 spectrophotometer. The spectrophotometric cell was irradiated in the center of a petri dish (6 × 12 cm) filled with a water–ice mixture and covered externally with aluminum foil. There was a 2-cm aperture on one side of the petri dish for the beam of a 150-W lamp. A 3-second irradiation saturated the phototransformation. The relative amount of PCH, subsequently called activity, was calculated from the absorption spectrum before irradiation. A straight line connecting absorbance values at 595 and 670 nm was the baseline. The activity of a preparation was defined as the volume in milliliters times the absorbance at 639 nm measured from the baseline. The yield, specific activity, the transformability of PCH was considerably enhanced by extracting the tissue with low concentrations, 0.05–0.1%, of Triton X-100." @default.
- W1695661112 created "2016-06-24" @default.
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- W1695661112 date "1971-01-01" @default.
- W1695661112 modified "2023-09-27" @default.
- W1695661112 title "[54] Protochlorophyllide holochrome" @default.
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- W1695661112 doi "https://doi.org/10.1016/s0076-6879(71)23130-x" @default.
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