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- W1695689747 abstract "This chapter discusses the preparation of phospholipids and their analogs by phospholipase D. Egg phosphatidylcholine was extracted from egg yolk and purified on silica gel 60, using solvent mixtures of chloroform-methanol-ammonia in ratios of 200:15:1 to start with and 65: 15:1 for the elution of egg phosphatidylcholine. The pure lipid dissolved in chloroform-methanol, 9:1 (by volume), was stored at 0° under nitrogen. The transfer of the phosphatidyl residue from phosphatidylcholine to primary alcohols or ethanolamines was detected by TLC in the solvent system chloroform-methanol-ammonia 65:30:3. The hydrolysis of phosphatidylethanolamines was performed in the presence of Triton X-100, which also facilitated the formation of clear emulsions and thus allowed complete hydrolysis of the substrates. It is found that no difference in the extent and rate of transphosphatidylation was observed when smaller samples were used in the experiments with respectively reduced amounts of enzyme and primary alkanol and volumes of the buffer–ether mixture." @default.
- W1695689747 created "2016-06-24" @default.
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- W1695689747 date "1981-01-01" @default.
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- W1695689747 title "[53] Preparation of phospholipids and their analogs by phospholipase D" @default.
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- W1695689747 doi "https://doi.org/10.1016/s0076-6879(81)72055-x" @default.
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