Matches in SemOpenAlex for { <https://semopenalex.org/work/W169574138> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W169574138 abstract "Trypanosomes are vector-borne parasitic haemoprotozoa that infect all classes of vertebrates, and are the etiological agents of severe diseases accompanied by a range of clinical signs including fatigue, fever, anaemia, and death, in both animals and humans1 . Little is known about the prevalence and pathogenesis of trypanosomes in Australian marsupials and monotremes, and few genetic characterisation studies have been conducted.During this study, using molecular and microscopic methods, we screened peripheral blood (n=27) and ectoparasites (n=8, from 7 animals) collected from wild Tasmanian platypuses (Ornithorhynchus anatinus), for the presence of trypanosomatid-specific DNA and trypanosomes. The genes for the small ribosomal subunit RNA (18S rDNA) and glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) were amplified and sequenced, prior to examining phylogenetic relationships by the maximum likelihood (ML) and neighbour joining (NJ) methods.Based on molecular results, parasite 18S rDNA prevalence was 37% (n=10/27) in blood samples. Additionally, one tick out of eight provided positive amplification and specific sequencing products, at the same locus. This apparently high prevalence was confirmed by the microscopy results, consistent with high parasitemia and the presence of abundant trypomastigotes in the blood films. Phylogenetic analyses at the 18S locus revealed the existence of multiple trypanosomatid-like genotypes, similar but distinct from the previously described Trypanosoma binneyi Mackerras, 19592 . The novel, statistically-supported clade (>85%), included new platypus-derived genotypes with 0.4-0.9% genetic distance from known T. binneyi sequences.The phylogenetic reconstructions of the trypanosomatid 18S rDNA, isolated from monotremes and marsupials3 , reveal a significant genetic diversity of the parasites associated with these unique hosts. The presence of various native Australian mammalian hosts, on multiple branches of the 18Sr rDNA tree of the monophyletic genus Trypanosoma, confirms the long evolutionary history of the parasite-host system, within the mammalian lineage." @default.
- W169574138 created "2016-06-24" @default.
- W169574138 creator A5008493746 @default.
- W169574138 creator A5033295595 @default.
- W169574138 creator A5047258052 @default.
- W169574138 creator A5085341585 @default.
- W169574138 date "2013-01-01" @default.
- W169574138 modified "2023-09-27" @default.
- W169574138 title "Trypanosomes from native Australian mammals: novel genotypes from the platypus (Ornithorhynchus anatinus)" @default.
- W169574138 hasPublicationYear "2013" @default.
- W169574138 type Work @default.
- W169574138 sameAs 169574138 @default.
- W169574138 citedByCount "0" @default.
- W169574138 crossrefType "journal-article" @default.
- W169574138 hasAuthorship W169574138A5008493746 @default.
- W169574138 hasAuthorship W169574138A5033295595 @default.
- W169574138 hasAuthorship W169574138A5047258052 @default.
- W169574138 hasAuthorship W169574138A5085341585 @default.
- W169574138 hasConcept C104317684 @default.
- W169574138 hasConcept C135763542 @default.
- W169574138 hasConcept C136764020 @default.
- W169574138 hasConcept C144024400 @default.
- W169574138 hasConcept C149923435 @default.
- W169574138 hasConcept C193252679 @default.
- W169574138 hasConcept C2777322942 @default.
- W169574138 hasConcept C2779549974 @default.
- W169574138 hasConcept C2908647359 @default.
- W169574138 hasConcept C41008148 @default.
- W169574138 hasConcept C44465124 @default.
- W169574138 hasConcept C47698986 @default.
- W169574138 hasConcept C54355233 @default.
- W169574138 hasConcept C67905577 @default.
- W169574138 hasConcept C71928629 @default.
- W169574138 hasConcept C78458016 @default.
- W169574138 hasConcept C81977670 @default.
- W169574138 hasConcept C84597430 @default.
- W169574138 hasConcept C86803240 @default.
- W169574138 hasConcept C90132467 @default.
- W169574138 hasConcept C90856448 @default.
- W169574138 hasConceptScore W169574138C104317684 @default.
- W169574138 hasConceptScore W169574138C135763542 @default.
- W169574138 hasConceptScore W169574138C136764020 @default.
- W169574138 hasConceptScore W169574138C144024400 @default.
- W169574138 hasConceptScore W169574138C149923435 @default.
- W169574138 hasConceptScore W169574138C193252679 @default.
- W169574138 hasConceptScore W169574138C2777322942 @default.
- W169574138 hasConceptScore W169574138C2779549974 @default.
- W169574138 hasConceptScore W169574138C2908647359 @default.
- W169574138 hasConceptScore W169574138C41008148 @default.
- W169574138 hasConceptScore W169574138C44465124 @default.
- W169574138 hasConceptScore W169574138C47698986 @default.
- W169574138 hasConceptScore W169574138C54355233 @default.
- W169574138 hasConceptScore W169574138C67905577 @default.
- W169574138 hasConceptScore W169574138C71928629 @default.
- W169574138 hasConceptScore W169574138C78458016 @default.
- W169574138 hasConceptScore W169574138C81977670 @default.
- W169574138 hasConceptScore W169574138C84597430 @default.
- W169574138 hasConceptScore W169574138C86803240 @default.
- W169574138 hasConceptScore W169574138C90132467 @default.
- W169574138 hasConceptScore W169574138C90856448 @default.
- W169574138 hasLocation W1695741381 @default.
- W169574138 hasOpenAccess W169574138 @default.
- W169574138 hasPrimaryLocation W1695741381 @default.
- W169574138 hasRelatedWork W1978170235 @default.
- W169574138 hasRelatedWork W1980937380 @default.
- W169574138 hasRelatedWork W2025679149 @default.
- W169574138 hasRelatedWork W2061154613 @default.
- W169574138 hasRelatedWork W2091505713 @default.
- W169574138 hasRelatedWork W2096107952 @default.
- W169574138 hasRelatedWork W2114479490 @default.
- W169574138 hasRelatedWork W2115079672 @default.
- W169574138 hasRelatedWork W2138309027 @default.
- W169574138 hasRelatedWork W2150222166 @default.
- W169574138 hasRelatedWork W2166071086 @default.
- W169574138 hasRelatedWork W2345463796 @default.
- W169574138 hasRelatedWork W2396233819 @default.
- W169574138 hasRelatedWork W2484044612 @default.
- W169574138 hasRelatedWork W2737293737 @default.
- W169574138 hasRelatedWork W2782779462 @default.
- W169574138 hasRelatedWork W2792987692 @default.
- W169574138 hasRelatedWork W3083512970 @default.
- W169574138 hasRelatedWork W2410362208 @default.
- W169574138 hasRelatedWork W2538049831 @default.
- W169574138 isParatext "false" @default.
- W169574138 isRetracted "false" @default.
- W169574138 magId "169574138" @default.
- W169574138 workType "article" @default.