Matches in SemOpenAlex for { <https://semopenalex.org/work/W169694512> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W169694512 abstract "Mature Smac, a homodimeric mitochondrial protein lacking the first 55 amino acids, exits mitochondria and promotes apoptosis by directly antagonizing IAPs. We used the ubiquitin fusion method to express mutant and wild type mature Smac in the cytosol of the 911 line of human embryonic retinoblasts. Mutations V78E, V81D, and F88D in the dimeric interface of Smac each prevented homodimerization, as determined by pull down of transiently expressed mutant Smac-V5 by transiently expressed mutant Smac-GST. Each of the Smac mutants also failed to heterodimerize with wild type Smac. We induced apoptosis in 911 cells that stably expressed cytosolic Smac by a 24 h treatment with zIEALal, a peptidyl aldehyde inhibitor of the proteasome. Apoptosis was measured by FACS analysis with annexin V PE and 7-AAD. Each of the monomeric Smac mutants potentiated the induction of apoptosis by zIEALal at least as well as wild type Smac. Caspase activation in 911 cells that stably expressed cytosolic Smac was demonstrated by western analysis of cleaved PARP after a 10 h zIEALal treatment. Cells that expressed the F88D-Smac mutant accumulated significantly more cleaved PARP than those that expressed wild type Smac. Interestingly, stable expression of wild type Smac in the cytosol of 911 cells markedly decreased its ability to interact with IAPs, which may be due to an as yet unidentified post-translational modification. Following transient expression in 911 cells, monomeric F88D-Smac failed to co-immunoprecipitate with any of the 6 IAPs tested (cIAP1, cIAP2, XIAP, Survivin, Livin, or Apollon). These findings suggest that mature Smac can exert a proapoptotic action that is independent of a direct interaction with IAPs. Supported by grant GM60383 from NIH." @default.
- W169694512 created "2016-06-24" @default.
- W169694512 creator A5006115526 @default.
- W169694512 creator A5050153212 @default.
- W169694512 date "2009-04-01" @default.
- W169694512 modified "2023-09-26" @default.
- W169694512 title "Cytosolic expression of dimerization defective mutants of mature Smac potentiate induction of apoptosis without interacting with inhibitor of apoptosis proteins (IAPs)" @default.
- W169694512 doi "https://doi.org/10.1096/fasebj.23.1_supplement.526.23" @default.
- W169694512 hasPublicationYear "2009" @default.
- W169694512 type Work @default.
- W169694512 sameAs 169694512 @default.
- W169694512 citedByCount "0" @default.
- W169694512 crossrefType "journal-article" @default.
- W169694512 hasAuthorship W169694512A5006115526 @default.
- W169694512 hasAuthorship W169694512A5050153212 @default.
- W169694512 hasConcept C104317684 @default.
- W169694512 hasConcept C143065580 @default.
- W169694512 hasConcept C153911025 @default.
- W169694512 hasConcept C170301204 @default.
- W169694512 hasConcept C181199279 @default.
- W169694512 hasConcept C185592680 @default.
- W169694512 hasConcept C190283241 @default.
- W169694512 hasConcept C207583985 @default.
- W169694512 hasConcept C2775975398 @default.
- W169694512 hasConcept C2776148510 @default.
- W169694512 hasConcept C2777408456 @default.
- W169694512 hasConcept C2778367456 @default.
- W169694512 hasConcept C31573885 @default.
- W169694512 hasConcept C33195913 @default.
- W169694512 hasConcept C55493867 @default.
- W169694512 hasConcept C71829478 @default.
- W169694512 hasConcept C86803240 @default.
- W169694512 hasConcept C95444343 @default.
- W169694512 hasConcept C98424977 @default.
- W169694512 hasConcept C98539663 @default.
- W169694512 hasConceptScore W169694512C104317684 @default.
- W169694512 hasConceptScore W169694512C143065580 @default.
- W169694512 hasConceptScore W169694512C153911025 @default.
- W169694512 hasConceptScore W169694512C170301204 @default.
- W169694512 hasConceptScore W169694512C181199279 @default.
- W169694512 hasConceptScore W169694512C185592680 @default.
- W169694512 hasConceptScore W169694512C190283241 @default.
- W169694512 hasConceptScore W169694512C207583985 @default.
- W169694512 hasConceptScore W169694512C2775975398 @default.
- W169694512 hasConceptScore W169694512C2776148510 @default.
- W169694512 hasConceptScore W169694512C2777408456 @default.
- W169694512 hasConceptScore W169694512C2778367456 @default.
- W169694512 hasConceptScore W169694512C31573885 @default.
- W169694512 hasConceptScore W169694512C33195913 @default.
- W169694512 hasConceptScore W169694512C55493867 @default.
- W169694512 hasConceptScore W169694512C71829478 @default.
- W169694512 hasConceptScore W169694512C86803240 @default.
- W169694512 hasConceptScore W169694512C95444343 @default.
- W169694512 hasConceptScore W169694512C98424977 @default.
- W169694512 hasConceptScore W169694512C98539663 @default.
- W169694512 hasFunder F4320332161 @default.
- W169694512 hasIssue "S1" @default.
- W169694512 hasLocation W1696945121 @default.
- W169694512 hasOpenAccess W169694512 @default.
- W169694512 hasPrimaryLocation W1696945121 @default.
- W169694512 hasRelatedWork W1509531702 @default.
- W169694512 hasRelatedWork W169694512 @default.
- W169694512 hasRelatedWork W1829810608 @default.
- W169694512 hasRelatedWork W1970081501 @default.
- W169694512 hasRelatedWork W2001919785 @default.
- W169694512 hasRelatedWork W2029107211 @default.
- W169694512 hasRelatedWork W2077168835 @default.
- W169694512 hasRelatedWork W2386192757 @default.
- W169694512 hasRelatedWork W2603253816 @default.
- W169694512 hasRelatedWork W3146483921 @default.
- W169694512 hasVolume "23" @default.
- W169694512 isParatext "false" @default.
- W169694512 isRetracted "false" @default.
- W169694512 magId "169694512" @default.
- W169694512 workType "article" @default.