FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1697904816> ?p ?o ?g. }

• W1697904816 endingPage "327" @default.
• W1697904816 startingPage "319" @default.
• W1697904816 abstract "The Escherichia coli pfkA gene has been cloned in the non-self-transmissible vector pVK101 from hybrid plasmids obtained from the Clarke and Carbon clone bank, resulting in the plasmids pAS300 and pAS100; the latter plasmid also encoded the E. coli tpi gene. These plasmids were transferred by conjugation to mutants of Alcaligenes eutrophus which are unable to grow on fructose and gluconate due to lack of 2-keto-3-deoxy-6-phosphogluconate aldolase activity. These transconjugants recovered the ability to grow on fructose and harbored pAS100 or pAS300. After growth on fructose, the transconjugants contained phosphofructokinase at specific activities between 0.73 and 1.83 U/mg of protein, indicating that the E. coli pfkA gene is readily expressed in A. eutrophus and that the utilization of fructose occurs via the Embden-Meyerhof pathway instead of the Entner-Doudoroff pathway. In contrast, transconjugants of the wild type of A. eutrophus, which are potentially able to catabolize fructose via both pathways, grew at a decreased rate on fructose and during growth on fructose did not stably maintain pAS100 or pAS300. Indications for a glycolytic futile cycling of fructose 6-phosphate and fructose 1,6-bisphosphate are discussed. Plasmid pA 100 was also transferred to 14 different species of gram-negative bacteria. The pfkA gene was expressed in most of these species. In addition, most transconjugants of these strains and of A. eutrophus exhibited higher specific activities of triosephosphate isomerase than did the corresponding parent strains." @default.
• W1697904816 created "2016-06-24" @default.
• W1697904816 creator A5047708437 @default.
• W1697904816 date "1986-04-01" @default.
• W1697904816 modified "2023-10-16" @default.
• W1697904816 title "Expression of the Escherichia coli pfkA gene in Alcaligenes eutrophus and in other gram-negative bacteria" @default.
• W1697904816 cites W1509509583 @default.
• W1697904816 cites W1512057211 @default.
• W1697904816 cites W1520468203 @default.
• W1697904816 cites W1546409791 @default.
• W1697904816 cites W1548594428 @default.
• W1697904816 cites W1585714582 @default.
• W1697904816 cites W1815658416 @default.
• W1697904816 cites W1837970756 @default.
• W1697904816 cites W1909460360 @default.
• W1697904816 cites W1920459436 @default.
• W1697904816 cites W1928366587 @default.
• W1697904816 cites W1951834406 @default.
• W1697904816 cites W1974530135 @default.
• W1697904816 cites W1998231415 @default.
• W1697904816 cites W2001651235 @default.
• W1697904816 cites W2009584069 @default.
• W1697904816 cites W2018396914 @default.
• W1697904816 cites W2019410656 @default.
• W1697904816 cites W2027229485 @default.
• W1697904816 cites W2044040104 @default.
• W1697904816 cites W2069573006 @default.
• W1697904816 cites W2080872979 @default.
• W1697904816 cites W2083213131 @default.
• W1697904816 cites W2087964217 @default.
• W1697904816 cites W2088263641 @default.
• W1697904816 cites W2088792381 @default.
• W1697904816 cites W2089138855 @default.
• W1697904816 cites W2111729024 @default.
• W1697904816 cites W2117277433 @default.
• W1697904816 cites W2127969464 @default.
• W1697904816 cites W2128635872 @default.
• W1697904816 cites W2141800449 @default.
• W1697904816 cites W2336061894 @default.
• W1697904816 cites W2417274144 @default.
• W1697904816 cites W2971321726 @default.
• W1697904816 cites W2997482688 @default.
• W1697904816 cites W3011914914 @default.
• W1697904816 cites W4293247451 @default.
• W1697904816 cites W53076314 @default.
• W1697904816 cites W96805779 @default.
• W1697904816 doi "https://doi.org/10.1128/jb.166.1.319-327.1986" @default.
• W1697904816 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/214595" @default.
• W1697904816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2937774" @default.
• W1697904816 hasPublicationYear "1986" @default.
• W1697904816 type Work @default.
• W1697904816 sameAs 1697904816 @default.
• W1697904816 citedByCount "19" @default.
• W1697904816 countsByYear W16979048162012 @default.
• W1697904816 countsByYear W16979048162013 @default.
• W1697904816 countsByYear W16979048162014 @default.
• W1697904816 countsByYear W16979048162015 @default.
• W1697904816 countsByYear W16979048162017 @default.
• W1697904816 countsByYear W16979048162021 @default.
• W1697904816 crossrefType "journal-article" @default.
• W1697904816 hasAuthorship W1697904816A5047708437 @default.
• W1697904816 hasBestOaLocation W16979048162 @default.
• W1697904816 hasConcept C104317684 @default.
• W1697904816 hasConcept C143065580 @default.
• W1697904816 hasConcept C181199279 @default.
• W1697904816 hasConcept C22744801 @default.
• W1697904816 hasConcept C2776970464 @default.
• W1697904816 hasConcept C2778910516 @default.
• W1697904816 hasConcept C2780199933 @default.
• W1697904816 hasConcept C2781463711 @default.
• W1697904816 hasConcept C28165981 @default.
• W1697904816 hasConcept C3488265 @default.
• W1697904816 hasConcept C523546767 @default.
• W1697904816 hasConcept C54355233 @default.
• W1697904816 hasConcept C547475151 @default.
• W1697904816 hasConcept C55493867 @default.
• W1697904816 hasConcept C86803240 @default.
• W1697904816 hasConcept C89423630 @default.
• W1697904816 hasConceptScore W1697904816C104317684 @default.
• W1697904816 hasConceptScore W1697904816C143065580 @default.
• W1697904816 hasConceptScore W1697904816C181199279 @default.
• W1697904816 hasConceptScore W1697904816C22744801 @default.
• W1697904816 hasConceptScore W1697904816C2776970464 @default.
• W1697904816 hasConceptScore W1697904816C2778910516 @default.
• W1697904816 hasConceptScore W1697904816C2780199933 @default.
• W1697904816 hasConceptScore W1697904816C2781463711 @default.
• W1697904816 hasConceptScore W1697904816C28165981 @default.
• W1697904816 hasConceptScore W1697904816C3488265 @default.
• W1697904816 hasConceptScore W1697904816C523546767 @default.
• W1697904816 hasConceptScore W1697904816C54355233 @default.
• W1697904816 hasConceptScore W1697904816C547475151 @default.
• W1697904816 hasConceptScore W1697904816C55493867 @default.
• W1697904816 hasConceptScore W1697904816C86803240 @default.
• W1697904816 hasConceptScore W1697904816C89423630 @default.
• W1697904816 hasIssue "1" @default.
• W1697904816 hasLocation W16979048161 @default.
• W1697904816 hasLocation W16979048162 @default.
• W1697904816 hasLocation W16979048163 @default.

• next