FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1698099488> ?p ?o ?g. }

• W1698099488 abstract "To construct a transgene expressing human endomorphin-2 by linking the signal peptide of mouse nerve growth factor (PN) to a human endomorphin-2 DNA sequence containing a short linker recognized by the protease FURIN and test the analgesic effect of endomorphin-2 on neuropathic pain.The transgene was inserted into the cosmid pAxCAwt to generate PN-EM-2-pAxCAwt. The recombinant adenovirus Ad-PNEM2 was packaged and propagated in HEK293 cells. After the Ad-PNEM2-infected NIH3T3 cells had been cultured, protein expression was examined by immunofluorescence and ELISA. A CCI rat model was constructed and the Ad-PNEM2 was administered intrathecally. The rats' pain thresholds (PWL) were measured and the presence of endomorphin-2 in the cerebrospinal fluid was confirmed through ELISA.The Ad-PNEM2 expressed endomorphin-2 smoothly and abundantly in NIH3T3 cells at a significantly higher rate than the viral control (P<0.01) or blank control (P<0.01). The expressed endomorphin-2 was mainly observed in the cytoplasm. The concentration of endomorphin-2 in the cerebrospinal fluid increased 1 day after injection and peaked between 7 and 14 days after injection. After injection, PWL approached normal levels in the operated study group. No significant change was observed in the control groups. There was a significant correlation between PWL and endomorphin-2 level (r = 0.944, P<0.001).The constructed human endomorphin-2 transgene was expressed effectively, and endomorphin-2 expressed by the recombinant adenovirus altered the threshold to thermal stimulus and showed good analgesic effect." @default.
• W1698099488 created "2016-06-24" @default.
• W1698099488 creator A5019425672 @default.
• W1698099488 creator A5024468354 @default.
• W1698099488 creator A5035355028 @default.
• W1698099488 creator A5046124767 @default.
• W1698099488 creator A5062509669 @default.
• W1698099488 creator A5087162426 @default.
• W1698099488 date "2012-01-01" @default.
• W1698099488 modified "2023-09-24" @default.
• W1698099488 title "Effect of recombinant adenovirus coding for endomorphin-2 on neuropathic pain in rats." @default.
• W1698099488 cites W1970892256 @default.
• W1698099488 cites W1971891998 @default.
• W1698099488 cites W1978329719 @default.
• W1698099488 cites W1983148368 @default.
• W1698099488 cites W1986751279 @default.
• W1698099488 cites W1994726921 @default.
• W1698099488 cites W2011973300 @default.
• W1698099488 cites W2019229473 @default.
• W1698099488 cites W2021307215 @default.
• W1698099488 cites W2038078632 @default.
• W1698099488 cites W2060971940 @default.
• W1698099488 cites W2064462697 @default.
• W1698099488 cites W2083411648 @default.
• W1698099488 cites W2084849531 @default.
• W1698099488 cites W2085426137 @default.
• W1698099488 cites W2086021531 @default.
• W1698099488 cites W2143729842 @default.
• W1698099488 cites W2150290162 @default.
• W1698099488 cites W2150857721 @default.
• W1698099488 cites W2162172962 @default.
• W1698099488 cites W2269897861 @default.
• W1698099488 cites W2292294584 @default.
• W1698099488 cites W2409759803 @default.
• W1698099488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3484491" @default.
• W1698099488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23119108" @default.
• W1698099488 hasPublicationYear "2012" @default.
• W1698099488 type Work @default.
• W1698099488 sameAs 1698099488 @default.
• W1698099488 citedByCount "3" @default.
• W1698099488 countsByYear W16980994882015 @default.
• W1698099488 countsByYear W16980994882016 @default.
• W1698099488 countsByYear W16980994882017 @default.
• W1698099488 crossrefType "journal-article" @default.
• W1698099488 hasAuthorship W1698099488A5019425672 @default.
• W1698099488 hasAuthorship W1698099488A5024468354 @default.
• W1698099488 hasAuthorship W1698099488A5035355028 @default.
• W1698099488 hasAuthorship W1698099488A5046124767 @default.
• W1698099488 hasAuthorship W1698099488A5062509669 @default.
• W1698099488 hasAuthorship W1698099488A5087162426 @default.
• W1698099488 hasConcept C102230213 @default.
• W1698099488 hasConcept C104317684 @default.
• W1698099488 hasConcept C126322002 @default.
• W1698099488 hasConcept C153911025 @default.
• W1698099488 hasConcept C170493617 @default.
• W1698099488 hasConcept C174510640 @default.
• W1698099488 hasConcept C185592680 @default.
• W1698099488 hasConcept C40767141 @default.
• W1698099488 hasConcept C55493867 @default.
• W1698099488 hasConcept C71924100 @default.
• W1698099488 hasConcept C86803240 @default.
• W1698099488 hasConcept C98274493 @default.
• W1698099488 hasConceptScore W1698099488C102230213 @default.
• W1698099488 hasConceptScore W1698099488C104317684 @default.
• W1698099488 hasConceptScore W1698099488C126322002 @default.
• W1698099488 hasConceptScore W1698099488C153911025 @default.
• W1698099488 hasConceptScore W1698099488C170493617 @default.
• W1698099488 hasConceptScore W1698099488C174510640 @default.
• W1698099488 hasConceptScore W1698099488C185592680 @default.
• W1698099488 hasConceptScore W1698099488C40767141 @default.
• W1698099488 hasConceptScore W1698099488C55493867 @default.
• W1698099488 hasConceptScore W1698099488C71924100 @default.
• W1698099488 hasConceptScore W1698099488C86803240 @default.
• W1698099488 hasConceptScore W1698099488C98274493 @default.
• W1698099488 hasLocation W16980994881 @default.
• W1698099488 hasOpenAccess W1698099488 @default.
• W1698099488 hasPrimaryLocation W16980994881 @default.
• W1698099488 hasRelatedWork W1608028227 @default.
• W1698099488 hasRelatedWork W1964708147 @default.
• W1698099488 hasRelatedWork W2033862675 @default.
• W1698099488 hasRelatedWork W2126232334 @default.
• W1698099488 hasRelatedWork W2171292840 @default.
• W1698099488 hasRelatedWork W2357591953 @default.
• W1698099488 hasRelatedWork W2359023584 @default.
• W1698099488 hasRelatedWork W2365681687 @default.
• W1698099488 hasRelatedWork W2365818615 @default.
• W1698099488 hasRelatedWork W2368923705 @default.
• W1698099488 hasRelatedWork W2370660859 @default.
• W1698099488 hasRelatedWork W2370727864 @default.
• W1698099488 hasRelatedWork W2373705192 @default.
• W1698099488 hasRelatedWork W2376078503 @default.
• W1698099488 hasRelatedWork W2382010748 @default.
• W1698099488 hasRelatedWork W2386286863 @default.
• W1698099488 hasRelatedWork W2409184855 @default.
• W1698099488 hasRelatedWork W2903282418 @default.
• W1698099488 hasRelatedWork W601646597 @default.
• W1698099488 hasRelatedWork W2576858051 @default.
• W1698099488 isParatext "false" @default.
• W1698099488 isRetracted "false" @default.
• W1698099488 magId "1698099488" @default.

• next