Matches in SemOpenAlex for { <https://semopenalex.org/work/W1698472007> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W1698472007 endingPage "3835" @default.
- W1698472007 startingPage "3830" @default.
- W1698472007 abstract "Abstract A 30,000 m.w. protein bound tightly to C5b6, which was formed by activating C7-depleted human serum with zymosan. The protein remained bound to the C5b6 complex during the isolation procedure for C5b6, including chromatography on lysine-Sepharose and an anion exchange resin. Following electrophoresis and electroblotting of the C5b6 complex to a polyvinylidene difluoride transfer membrane, the 30,000 m.w. protein was microsequenced. The 24 N-terminal amino acid sequence was determined and showed identity of the 30,000 m.w. protein with the serum amyloid P (SAP) component. The C5b6-SAP complex did not dissociate in the presence of 10 mM EDTA, which distinguishes SAP-C5b6 binding from SAP's usual Ca2+-dependent binding to other molecules. SAP, which was isolated from serum by chromatography, was able to bind to preformed C5b6, which had been assembled and isolated from purified components. Functionally, the C5b6-SAP could bind C7, and the resulting C5b67-SAP complex had only moderately lower specific hemolytic activity than that of C5b67. In addition, hemolytically inactive C5b67-SAP, such as hemolytically inactive C5b67, was chemotactically active for neutrophils, while isolated SAP had no effect on cell mobility. Because SAP reacts with other serum proteins and with cells, it is likely that the addition of SAP to terminal complement complexes may affect the fate of these complexes." @default.
- W1698472007 created "2016-06-24" @default.
- W1698472007 creator A5012886188 @default.
- W1698472007 creator A5046736628 @default.
- W1698472007 creator A5068931327 @default.
- W1698472007 date "1997-04-15" @default.
- W1698472007 modified "2023-10-02" @default.
- W1698472007 title "Serum amyloid P component forms a stable complex with human C5b6." @default.
- W1698472007 doi "https://doi.org/10.4049/jimmunol.158.8.3830" @default.
- W1698472007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9103450" @default.
- W1698472007 hasPublicationYear "1997" @default.
- W1698472007 type Work @default.
- W1698472007 sameAs 1698472007 @default.
- W1698472007 citedByCount "7" @default.
- W1698472007 countsByYear W16984720072012 @default.
- W1698472007 countsByYear W16984720072018 @default.
- W1698472007 crossrefType "journal-article" @default.
- W1698472007 hasAuthorship W1698472007A5012886188 @default.
- W1698472007 hasAuthorship W1698472007A5046736628 @default.
- W1698472007 hasAuthorship W1698472007A5068931327 @default.
- W1698472007 hasBestOaLocation W16984720071 @default.
- W1698472007 hasConcept C101660505 @default.
- W1698472007 hasConcept C104317684 @default.
- W1698472007 hasConcept C149232098 @default.
- W1698472007 hasConcept C179247698 @default.
- W1698472007 hasConcept C181199279 @default.
- W1698472007 hasConcept C185592680 @default.
- W1698472007 hasConcept C203014093 @default.
- W1698472007 hasConcept C2776515853 @default.
- W1698472007 hasConcept C2776914184 @default.
- W1698472007 hasConcept C2779201268 @default.
- W1698472007 hasConcept C2779881004 @default.
- W1698472007 hasConcept C2780029684 @default.
- W1698472007 hasConcept C2781195455 @default.
- W1698472007 hasConcept C553089730 @default.
- W1698472007 hasConcept C55493867 @default.
- W1698472007 hasConcept C86803240 @default.
- W1698472007 hasConceptScore W1698472007C101660505 @default.
- W1698472007 hasConceptScore W1698472007C104317684 @default.
- W1698472007 hasConceptScore W1698472007C149232098 @default.
- W1698472007 hasConceptScore W1698472007C179247698 @default.
- W1698472007 hasConceptScore W1698472007C181199279 @default.
- W1698472007 hasConceptScore W1698472007C185592680 @default.
- W1698472007 hasConceptScore W1698472007C203014093 @default.
- W1698472007 hasConceptScore W1698472007C2776515853 @default.
- W1698472007 hasConceptScore W1698472007C2776914184 @default.
- W1698472007 hasConceptScore W1698472007C2779201268 @default.
- W1698472007 hasConceptScore W1698472007C2779881004 @default.
- W1698472007 hasConceptScore W1698472007C2780029684 @default.
- W1698472007 hasConceptScore W1698472007C2781195455 @default.
- W1698472007 hasConceptScore W1698472007C553089730 @default.
- W1698472007 hasConceptScore W1698472007C55493867 @default.
- W1698472007 hasConceptScore W1698472007C86803240 @default.
- W1698472007 hasIssue "8" @default.
- W1698472007 hasLocation W16984720071 @default.
- W1698472007 hasLocation W16984720072 @default.
- W1698472007 hasOpenAccess W1698472007 @default.
- W1698472007 hasPrimaryLocation W16984720071 @default.
- W1698472007 hasRelatedWork W1482247890 @default.
- W1698472007 hasRelatedWork W1698472007 @default.
- W1698472007 hasRelatedWork W1972819401 @default.
- W1698472007 hasRelatedWork W1978991067 @default.
- W1698472007 hasRelatedWork W1989751382 @default.
- W1698472007 hasRelatedWork W2003154045 @default.
- W1698472007 hasRelatedWork W2040849321 @default.
- W1698472007 hasRelatedWork W2088042652 @default.
- W1698472007 hasRelatedWork W2248267635 @default.
- W1698472007 hasRelatedWork W2325727162 @default.
- W1698472007 hasVolume "158" @default.
- W1698472007 isParatext "false" @default.
- W1698472007 isRetracted "false" @default.
- W1698472007 magId "1698472007" @default.
- W1698472007 workType "article" @default.