FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1699172361> ?p ?o ?g. }

• W1699172361 endingPage "3530" @default.
• W1699172361 startingPage "3521" @default.
• W1699172361 abstract "Inferred amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from five different methanogen species were aligned and two regions with a high degree of homology flanking a more variable region were identified. Analysis of the DNA sequences from the conserved regions yielded two degenerate sequences from which a forward primer, a 32-mer, and a reverse primer, a 23-mer, could be derived for use in the specific PCR-based detection of methanogens. The primers were successfully evaluated against 23 species of methanogen representing all five recognized orders of this group of Archaea, generating a PCR product between 464 and 491 bp. Comparisons between the mcrA and 16S small subunit rRNA gene sequences using PHYLIP demonstrated that the tree topologies were strikingly similar. Methods were developed to enable the analysis of methanogen populations in landfill using the mcrA gene as the target. Two landfill sites were examined and 63 clones from a site in Mucking, Essex, and 102 from a site in Odcombe, Somerset, were analysed. Analysis revealed a far greater diversity in the methanogen population within landfill material than has been seen previously." @default.
• W1699172361 created "2016-06-24" @default.
• W1699172361 creator A5015751915 @default.
• W1699172361 creator A5066770003 @default.
• W1699172361 creator A5067271602 @default.
• W1699172361 creator A5073084318 @default.
• W1699172361 date "2002-11-01" @default.
• W1699172361 modified "2023-10-10" @default.
• W1699172361 title "The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill b bThe GenBank accession numbers for the mcrA sequences reported in this paper are AF414034–AF414051 (see Fig. 2) and AF414007–AF414033 (environmental isolates in Fig. 3)." @default.
• W1699172361 cites W107663980 @default.
• W1699172361 cites W1520047635 @default.
• W1699172361 cites W1533991874 @default.
• W1699172361 cites W1547704153 @default.
• W1699172361 cites W1588533534 @default.
• W1699172361 cites W1591493785 @default.
• W1699172361 cites W1829963559 @default.
• W1699172361 cites W1838273421 @default.
• W1699172361 cites W1980207689 @default.
• W1699172361 cites W1988988120 @default.
• W1699172361 cites W2022916467 @default.
• W1699172361 cites W2032517801 @default.
• W1699172361 cites W2049253389 @default.
• W1699172361 cites W2062754790 @default.
• W1699172361 cites W2063411382 @default.
• W1699172361 cites W2066684304 @default.
• W1699172361 cites W2099124655 @default.
• W1699172361 cites W2109864391 @default.
• W1699172361 cites W2111271447 @default.
• W1699172361 cites W2121710598 @default.
• W1699172361 cites W2123584253 @default.
• W1699172361 cites W2134175807 @default.
• W1699172361 cites W2135784927 @default.
• W1699172361 cites W2154347365 @default.
• W1699172361 cites W2156465688 @default.
• W1699172361 cites W2159748283 @default.
• W1699172361 cites W2162017063 @default.
• W1699172361 cites W2162578061 @default.
• W1699172361 cites W2168511828 @default.
• W1699172361 cites W4236085443 @default.
• W1699172361 doi "https://doi.org/10.1099/00221287-148-11-3521" @default.
• W1699172361 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12427943" @default.
• W1699172361 hasPublicationYear "2002" @default.
• W1699172361 type Work @default.
• W1699172361 sameAs 1699172361 @default.
• W1699172361 citedByCount "746" @default.
• W1699172361 countsByYear W16991723612012 @default.
• W1699172361 countsByYear W16991723612013 @default.
• W1699172361 countsByYear W16991723612014 @default.
• W1699172361 countsByYear W16991723612015 @default.
• W1699172361 countsByYear W16991723612016 @default.
• W1699172361 countsByYear W16991723612017 @default.
• W1699172361 countsByYear W16991723612018 @default.
• W1699172361 countsByYear W16991723612019 @default.
• W1699172361 countsByYear W16991723612020 @default.
• W1699172361 countsByYear W16991723612021 @default.
• W1699172361 countsByYear W16991723612022 @default.
• W1699172361 countsByYear W16991723612023 @default.
• W1699172361 crossrefType "journal-article" @default.
• W1699172361 hasAuthorship W1699172361A5015751915 @default.
• W1699172361 hasAuthorship W1699172361A5066770003 @default.
• W1699172361 hasAuthorship W1699172361A5067271602 @default.
• W1699172361 hasAuthorship W1699172361A5073084318 @default.
• W1699172361 hasBestOaLocation W16991723611 @default.
• W1699172361 hasConcept C104317684 @default.
• W1699172361 hasConcept C144024400 @default.
• W1699172361 hasConcept C149923435 @default.
• W1699172361 hasConcept C178790620 @default.
• W1699172361 hasConcept C185592680 @default.
• W1699172361 hasConcept C193252679 @default.
• W1699172361 hasConcept C2777438225 @default.
• W1699172361 hasConcept C2777563447 @default.
• W1699172361 hasConcept C2908647359 @default.
• W1699172361 hasConcept C42062724 @default.
• W1699172361 hasConcept C523546767 @default.
• W1699172361 hasConcept C54355233 @default.
• W1699172361 hasConcept C550995028 @default.
• W1699172361 hasConcept C67905577 @default.
• W1699172361 hasConcept C79029880 @default.
• W1699172361 hasConcept C86803240 @default.
• W1699172361 hasConceptScore W1699172361C104317684 @default.
• W1699172361 hasConceptScore W1699172361C144024400 @default.
• W1699172361 hasConceptScore W1699172361C149923435 @default.
• W1699172361 hasConceptScore W1699172361C178790620 @default.
• W1699172361 hasConceptScore W1699172361C185592680 @default.
• W1699172361 hasConceptScore W1699172361C193252679 @default.
• W1699172361 hasConceptScore W1699172361C2777438225 @default.
• W1699172361 hasConceptScore W1699172361C2777563447 @default.
• W1699172361 hasConceptScore W1699172361C2908647359 @default.
• W1699172361 hasConceptScore W1699172361C42062724 @default.
• W1699172361 hasConceptScore W1699172361C523546767 @default.
• W1699172361 hasConceptScore W1699172361C54355233 @default.
• W1699172361 hasConceptScore W1699172361C550995028 @default.
• W1699172361 hasConceptScore W1699172361C67905577 @default.
• W1699172361 hasConceptScore W1699172361C79029880 @default.
• W1699172361 hasConceptScore W1699172361C86803240 @default.
• W1699172361 hasIssue "11" @default.
• W1699172361 hasLocation W16991723611 @default.
• W1699172361 hasLocation W16991723612 @default.

• next