FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1699485873> ?p ?o ?g. }

• W1699485873 abstract "Nucleic acids are a uniquely flexible and multi-faceted class of molecules that fulfill fundamental and defining tasks such as replication and determination of heritable characteristics in every living organism. From the microscopic to the gigantic, from the most primitive to the most complex, life has been both molded and served by nucleic acids. Nucleic acid circuits straddle the realm of nature and technology. The elegance of interaction between nucleic acid molecules invites us to gain a deeper understanding of the naturally occurring systems they compose and to apply our ingenuity and foresight toward developing ever more complex synthetic systems. Nature has provided these basic building blocks, which we can now arrange – and augment – for the purpose of creating molecular-level machinery.Here we describe some ways in which we have rationally harnessed nucleic acids. In preparation for outbreaks of novel and deadly avian influenza viruses, we used quantitative polymerase chain reaction (qPCR) to track the number of flu virus particles surviving in the presence of potential antiviral drugs. We engineered tunable on/off switches that can be used to evaluate a series of conditions for diagnostic applications or to enable ‘smart’ drugs that sense, analyze, and respond to their microenvironment.We optimized the conditions for, and used, a unique set of guanine-rich DNA sequences called G-quadruplexes, whose enzymatic and structural properties make them prime effector candidates in diagnostic platforms. G-quadruplex folding powers isothermal DNA amplification, and the small organic molecules they bind endow G-quadruplexes with expanded catalytic abilities. We genotyped drug resistance mutations in tuberculosis via visually detectable color changes in the reaction buffer. We developed a paper fluidics assay that employs soluble and bead-immobilized nucleic acids to scan for genes in tuberculosis, and upon detection, to generate a readily observable discoloration on the paper strip.Finally, we probed the boundary of nucleic acid circuitry by attempting to expand its language via the incorporation of unnatural nucleobases into oligonucleotide components of a catalytic hairpin assembly (CHA) circuit. We subsequently evaluated the resilience of the unnatural CHA circuit to contamination by random DNA species, such as may be encountered in clinical samples." @default.
• W1699485873 created "2016-06-24" @default.
• W1699485873 creator A5015588274 @default.
• W1699485873 date "2013-08-01" @default.
• W1699485873 modified "2023-09-27" @default.
• W1699485873 title "DNA-based molecular circuits for diagnostics and therapeutics" @default.
• W1699485873 cites W126601421 @default.
• W1699485873 cites W1481959267 @default.
• W1699485873 cites W1650197172 @default.
• W1699485873 cites W1767389458 @default.
• W1699485873 cites W1899305737 @default.
• W1699485873 cites W1963613325 @default.
• W1699485873 cites W1963880900 @default.
• W1699485873 cites W1964928696 @default.
• W1699485873 cites W1966034229 @default.
• W1699485873 cites W1966300154 @default.
• W1699485873 cites W1973503801 @default.
• W1699485873 cites W1980301976 @default.
• W1699485873 cites W1981582209 @default.
• W1699485873 cites W1986463454 @default.
• W1699485873 cites W1989044021 @default.
• W1699485873 cites W1990545448 @default.
• W1699485873 cites W1991297120 @default.
• W1699485873 cites W1991335064 @default.
• W1699485873 cites W1991933856 @default.
• W1699485873 cites W1992479402 @default.
• W1699485873 cites W1997027430 @default.
• W1699485873 cites W1999352184 @default.
• W1699485873 cites W2003926950 @default.
• W1699485873 cites W2008041430 @default.
• W1699485873 cites W2015503736 @default.
• W1699485873 cites W2027497473 @default.
• W1699485873 cites W2029582111 @default.
• W1699485873 cites W2035497135 @default.
• W1699485873 cites W2038929178 @default.
• W1699485873 cites W2046342455 @default.
• W1699485873 cites W2047155236 @default.
• W1699485873 cites W2050717506 @default.
• W1699485873 cites W2055658606 @default.
• W1699485873 cites W2056823236 @default.
• W1699485873 cites W2058144955 @default.
• W1699485873 cites W2060938911 @default.
• W1699485873 cites W2063901213 @default.
• W1699485873 cites W2066409539 @default.
• W1699485873 cites W2068743423 @default.
• W1699485873 cites W2069468367 @default.
• W1699485873 cites W2076728019 @default.
• W1699485873 cites W2081109112 @default.
• W1699485873 cites W2082027989 @default.
• W1699485873 cites W2082089611 @default.
• W1699485873 cites W2090341123 @default.
• W1699485873 cites W2093413494 @default.
• W1699485873 cites W2098076879 @default.
• W1699485873 cites W2098686081 @default.
• W1699485873 cites W2101111823 @default.
• W1699485873 cites W2102487925 @default.
• W1699485873 cites W2104525153 @default.
• W1699485873 cites W2104694288 @default.
• W1699485873 cites W2105944077 @default.
• W1699485873 cites W2106845910 @default.
• W1699485873 cites W2107263644 @default.
• W1699485873 cites W2108039757 @default.
• W1699485873 cites W2109010167 @default.
• W1699485873 cites W2114163400 @default.
• W1699485873 cites W2115434559 @default.
• W1699485873 cites W2117200691 @default.
• W1699485873 cites W2120271649 @default.
• W1699485873 cites W2120595078 @default.
• W1699485873 cites W2122139577 @default.
• W1699485873 cites W2123316289 @default.
• W1699485873 cites W2127306596 @default.
• W1699485873 cites W2129954525 @default.
• W1699485873 cites W2135542964 @default.
• W1699485873 cites W2139765356 @default.
• W1699485873 cites W2143784163 @default.
• W1699485873 cites W2145815431 @default.
• W1699485873 cites W2147608873 @default.
• W1699485873 cites W2149087734 @default.
• W1699485873 cites W2149793516 @default.
• W1699485873 cites W2150512887 @default.
• W1699485873 cites W2152599805 @default.
• W1699485873 cites W2153700418 @default.
• W1699485873 cites W2154288940 @default.
• W1699485873 cites W2156140162 @default.
• W1699485873 cites W2160763121 @default.
• W1699485873 cites W2168621505 @default.
• W1699485873 cites W2318322546 @default.
• W1699485873 cites W2400250419 @default.
• W1699485873 cites W2400315638 @default.
• W1699485873 cites W2422947363 @default.
• W1699485873 cites W28883393 @default.
• W1699485873 hasPublicationYear "2013" @default.
• W1699485873 type Work @default.
• W1699485873 sameAs 1699485873 @default.
• W1699485873 citedByCount "0" @default.
• W1699485873 crossrefType "journal-article" @default.
• W1699485873 hasAuthorship W1699485873A5015588274 @default.
• W1699485873 hasConcept C171250308 @default.
• W1699485873 hasConcept C185592680 @default.
• W1699485873 hasConcept C192562407 @default.

• next