FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W170626128> ?p ?o ?g. }

• W170626128 abstract "Monoclonal antibodies 2F5 and 4E 10 neutralize a broad range of HIV1 isolates. The epitopes for both antibodies are located in the membrane proximal region (MPR) of gp41, which is highly conserved among HIV-1 isolates. Attempts to use peptides or fusion proteins bearing the MPR as vaccines have failed, in that they produce antibodies that bind to gp41, but do not neutralize HIV1. Thus, 2F5 and 4E10 are unique among MPR-binding antibodies in their ability to neutralize the virus. The purpose of the work presented here is to identify peptides that are specific for 4E10, with the goal of producing a vaccine that elicits antibodies having 4ElO's unique, virus-neutralizing properties. A panel of phage-displayed random peptide libraries was screened with 4E10, and two distinct peptides were identified. One is a peptide that is homologous to the native 4E10 epitope in the MPR, and the other is a peptide that bears no homology to the MPR. Binding of these peptides to 4E10 was optimized through the construction of two libraries that are based on each peptide's sequence. Clones isolated from both libraries bound more strongly to 4ElO than to the corresponding parental clone. Alignment of the peptide sequences from multiple clones isolated from the linear doped library revealed shared residues that are presumably important for binding. The consensus sequence they form aligns with the previously mapped 4ElO epitope in the MPR, and extends beyond that sequence. The cyclic peptide provides an alternative mechanism of binding to 4E10, and as a ligand, may reveal its unique structural features. Both the linear and cyclic peptides are specific markers for 4E10, as sera of HIV1 infected people that bind to the 4ElO MPR epitope do not bind to either the linear or cyclic peptides. Thus, two types of peptide, which bind tightly and specifically to the neutralizing MAb 4E10, have been isolated and optimized. They have revealed the MPR epitope for 4ElO in more detail, and have served as specific serum markers for 4ElO. In future, they will be tested as leads for a vaccine that targets the production of 4E10-like antibodies." @default.
• W170626128 created "2016-06-24" @default.
• W170626128 creator A5029197404 @default.
• W170626128 date "2004-01-01" @default.
• W170626128 modified "2023-09-27" @default.
• W170626128 title "Peptide markers for the HIV-1 neutralizing antibody 4E10" @default.
• W170626128 cites W1506369361 @default.
• W170626128 cites W1517594500 @default.
• W170626128 cites W1528520360 @default.
• W170626128 cites W1528872128 @default.
• W170626128 cites W1532225053 @default.
• W170626128 cites W1534972788 @default.
• W170626128 cites W1591942886 @default.
• W170626128 cites W1605208680 @default.
• W170626128 cites W1635348995 @default.
• W170626128 cites W1645633783 @default.
• W170626128 cites W16961449 @default.
• W170626128 cites W1842798465 @default.
• W170626128 cites W1966261980 @default.
• W170626128 cites W1966616903 @default.
• W170626128 cites W1968648567 @default.
• W170626128 cites W1970409794 @default.
• W170626128 cites W1972254082 @default.
• W170626128 cites W1972937369 @default.
• W170626128 cites W1974092202 @default.
• W170626128 cites W1974152659 @default.
• W170626128 cites W1975085160 @default.
• W170626128 cites W1976901188 @default.
• W170626128 cites W1979383678 @default.
• W170626128 cites W1988257424 @default.
• W170626128 cites W1994322627 @default.
• W170626128 cites W1995355944 @default.
• W170626128 cites W1997677784 @default.
• W170626128 cites W1998987175 @default.
• W170626128 cites W2003157632 @default.
• W170626128 cites W2003760111 @default.
• W170626128 cites W2006582130 @default.
• W170626128 cites W2008606951 @default.
• W170626128 cites W2009371092 @default.
• W170626128 cites W2012362118 @default.
• W170626128 cites W2013835478 @default.
• W170626128 cites W2021346118 @default.
• W170626128 cites W2027738518 @default.
• W170626128 cites W2032380264 @default.
• W170626128 cites W2034160159 @default.
• W170626128 cites W2034399323 @default.
• W170626128 cites W2055834697 @default.
• W170626128 cites W2056802413 @default.
• W170626128 cites W2057246952 @default.
• W170626128 cites W2060679290 @default.
• W170626128 cites W2062947579 @default.
• W170626128 cites W2063701397 @default.
• W170626128 cites W2071914798 @default.
• W170626128 cites W2072925709 @default.
• W170626128 cites W2075964526 @default.
• W170626128 cites W2076789803 @default.
• W170626128 cites W2079163439 @default.
• W170626128 cites W2085528829 @default.
• W170626128 cites W2091209917 @default.
• W170626128 cites W2092441129 @default.
• W170626128 cites W2096387523 @default.
• W170626128 cites W2102548388 @default.
• W170626128 cites W2103156261 @default.
• W170626128 cites W2105789040 @default.
• W170626128 cites W2107768381 @default.
• W170626128 cites W2110287237 @default.
• W170626128 cites W2112353967 @default.
• W170626128 cites W2118820599 @default.
• W170626128 cites W2119460480 @default.
• W170626128 cites W2120161758 @default.
• W170626128 cites W2120653364 @default.
• W170626128 cites W2124445678 @default.
• W170626128 cites W2125231927 @default.
• W170626128 cites W2126977955 @default.
• W170626128 cites W2129274180 @default.
• W170626128 cites W2130455364 @default.
• W170626128 cites W2132895335 @default.
• W170626128 cites W2134419825 @default.
• W170626128 cites W2138551719 @default.
• W170626128 cites W2141444589 @default.
• W170626128 cites W2141679561 @default.
• W170626128 cites W2143312620 @default.
• W170626128 cites W2144831378 @default.
• W170626128 cites W2144975742 @default.
• W170626128 cites W2148366966 @default.
• W170626128 cites W2151364638 @default.
• W170626128 cites W2155429177 @default.
• W170626128 cites W2157272520 @default.
• W170626128 cites W2158181429 @default.
• W170626128 cites W2160959707 @default.
• W170626128 cites W2162546635 @default.
• W170626128 cites W2164060472 @default.
• W170626128 cites W2167627090 @default.
• W170626128 cites W2168357995 @default.
• W170626128 cites W2172063653 @default.
• W170626128 cites W2177434887 @default.
• W170626128 cites W2329327724 @default.
• W170626128 cites W2339006876 @default.
• W170626128 cites W2409720954 @default.
• W170626128 cites W35389034 @default.

• next