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- W1707171020 abstract "We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 <TEX>$mu$</TEX>g immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device." @default.
- W1707171020 created "2016-06-24" @default.
- W1707171020 date "2006-04-20" @default.
- W1707171020 modified "2023-10-18" @default.
- W1707171020 title "Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7" @default.
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- W1707171020 doi "https://doi.org/10.5012/bkcs.2006.27.4.479" @default.
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