Matches in SemOpenAlex for { <https://semopenalex.org/work/W1707726744> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1707726744 endingPage "90" @default.
- W1707726744 startingPage "84" @default.
- W1707726744 abstract "We report the bacterial expression and the purification of a monoclonal antibody (mAb) specific for an epitope that coincides with the LDL receptor (LDLr)-binding domain of human apolipoprotein E (apoE). This antibody resembles the LDLr in its primary structure and its specificity for apoE variants. The recombinant Fab (rFab) fragment of mAb 2E8, consisting of the entire light chain and the Fd portion of the heavy chain, was expressed inEscherichia coliand purified to homogeneity. Purification was facilitated by including a five-histidine carboxyl-terminal extension on the Fd chain. A 5- to 10-fold difference in yield of the antibody was observed when the plasmid was expressed in two different strains ofE. coli.Typically 2–6 mg of rFab per liter of culture medium was recovered in the periplasm of the TG1 strain and less than 1 mg was recovered in the periplasm of the XL1-Blue strain. Culture temperatures above 35°C or inclusion of sucrose in the medium reduced rFab yields. The 2E8 rFab was indistinguishable from Fab prepared from 2E8 hybridoma-generated IgG with respect to its affinity and fine specificity. We are using this system to express a panel of 2E8 variant Fabs that will be used as probes to establish the structural features responsible for the binding of apoE to the LDLr." @default.
- W1707726744 created "2016-06-24" @default.
- W1707726744 creator A5001672785 @default.
- W1707726744 creator A5008438859 @default.
- W1707726744 creator A5011302939 @default.
- W1707726744 creator A5033190041 @default.
- W1707726744 creator A5078761175 @default.
- W1707726744 creator A5084379540 @default.
- W1707726744 date "1999-06-01" @default.
- W1707726744 modified "2023-10-02" @default.
- W1707726744 title "Bacterial Expression and Purification of the Fab Fragment of a Monoclonal Antibody Specific for the Low-Density Lipoprotein Receptor-Binding Site of Human Apolipoprotein E" @default.
- W1707726744 cites W1989233913 @default.
- W1707726744 cites W1994543234 @default.
- W1707726744 cites W2006968970 @default.
- W1707726744 cites W2034968148 @default.
- W1707726744 cites W2040331602 @default.
- W1707726744 cites W2054774755 @default.
- W1707726744 cites W2061530347 @default.
- W1707726744 cites W2062929870 @default.
- W1707726744 cites W2066644955 @default.
- W1707726744 cites W2071378581 @default.
- W1707726744 cites W2112249914 @default.
- W1707726744 cites W2112251329 @default.
- W1707726744 cites W300404587 @default.
- W1707726744 cites W98152484 @default.
- W1707726744 doi "https://doi.org/10.1006/prep.1999.1031" @default.
- W1707726744 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10336864" @default.
- W1707726744 hasPublicationYear "1999" @default.
- W1707726744 type Work @default.
- W1707726744 sameAs 1707726744 @default.
- W1707726744 citedByCount "13" @default.
- W1707726744 crossrefType "journal-article" @default.
- W1707726744 hasAuthorship W1707726744A5001672785 @default.
- W1707726744 hasAuthorship W1707726744A5008438859 @default.
- W1707726744 hasAuthorship W1707726744A5011302939 @default.
- W1707726744 hasAuthorship W1707726744A5033190041 @default.
- W1707726744 hasAuthorship W1707726744A5078761175 @default.
- W1707726744 hasAuthorship W1707726744A5084379540 @default.
- W1707726744 hasConcept C104317684 @default.
- W1707726744 hasConcept C153911025 @default.
- W1707726744 hasConcept C159654299 @default.
- W1707726744 hasConcept C185592680 @default.
- W1707726744 hasConcept C195616568 @default.
- W1707726744 hasConcept C201663137 @default.
- W1707726744 hasConcept C203014093 @default.
- W1707726744 hasConcept C203750385 @default.
- W1707726744 hasConcept C2778163477 @default.
- W1707726744 hasConcept C2780072125 @default.
- W1707726744 hasConcept C40767141 @default.
- W1707726744 hasConcept C43554185 @default.
- W1707726744 hasConcept C542903549 @default.
- W1707726744 hasConcept C547475151 @default.
- W1707726744 hasConcept C55493867 @default.
- W1707726744 hasConcept C62746215 @default.
- W1707726744 hasConcept C86803240 @default.
- W1707726744 hasConceptScore W1707726744C104317684 @default.
- W1707726744 hasConceptScore W1707726744C153911025 @default.
- W1707726744 hasConceptScore W1707726744C159654299 @default.
- W1707726744 hasConceptScore W1707726744C185592680 @default.
- W1707726744 hasConceptScore W1707726744C195616568 @default.
- W1707726744 hasConceptScore W1707726744C201663137 @default.
- W1707726744 hasConceptScore W1707726744C203014093 @default.
- W1707726744 hasConceptScore W1707726744C203750385 @default.
- W1707726744 hasConceptScore W1707726744C2778163477 @default.
- W1707726744 hasConceptScore W1707726744C2780072125 @default.
- W1707726744 hasConceptScore W1707726744C40767141 @default.
- W1707726744 hasConceptScore W1707726744C43554185 @default.
- W1707726744 hasConceptScore W1707726744C542903549 @default.
- W1707726744 hasConceptScore W1707726744C547475151 @default.
- W1707726744 hasConceptScore W1707726744C55493867 @default.
- W1707726744 hasConceptScore W1707726744C62746215 @default.
- W1707726744 hasConceptScore W1707726744C86803240 @default.
- W1707726744 hasIssue "1" @default.
- W1707726744 hasLocation W17077267441 @default.
- W1707726744 hasLocation W17077267442 @default.
- W1707726744 hasOpenAccess W1707726744 @default.
- W1707726744 hasPrimaryLocation W17077267441 @default.
- W1707726744 hasRelatedWork W1977730060 @default.
- W1707726744 hasRelatedWork W1998990144 @default.
- W1707726744 hasRelatedWork W2013615486 @default.
- W1707726744 hasRelatedWork W2025216671 @default.
- W1707726744 hasRelatedWork W2039736738 @default.
- W1707726744 hasRelatedWork W2078659469 @default.
- W1707726744 hasRelatedWork W2092195735 @default.
- W1707726744 hasRelatedWork W2167581539 @default.
- W1707726744 hasRelatedWork W2182360597 @default.
- W1707726744 hasRelatedWork W2565798303 @default.
- W1707726744 hasVolume "16" @default.
- W1707726744 isParatext "false" @default.
- W1707726744 isRetracted "false" @default.
- W1707726744 magId "1707726744" @default.
- W1707726744 workType "article" @default.