Matches in SemOpenAlex for { <https://semopenalex.org/work/W1710233745> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1710233745 endingPage "202" @default.
- W1710233745 startingPage "1196" @default.
- W1710233745 abstract "Nitrogen dioxide (NO2), an environmental oxidant pollutant, is toxic to lung cells. One of the postulated mechanisms of NO2-induced pulmonary injury involves peroxidation of membrane lipids. Therefore, we evaluated the effect of 5 ppm NO2 exposure on membrane lipid fluidity, uptake of 5-hydroxytryptamine (5-HT), lactate dehydrogenase (LDH) release, and formation of lipid peroxides in porcine pulmonary artery and aortic endothelial cells in culture. After 3- to 24-h exposure, cells were labeled with 1,6-diphenyl-1,3,5-hexatriene (DPH), an aromatic hydrocarbon that partitions into the hydrophobic interior of the lipid bilayer of cell membranes. Membrane fluidity was monitored by measuring changes in rotational relaxation time (rho) for DPH by fluorescence spectroscopy. Reductions in membrane fluidity increase the value of rho. The 5-HT uptake was calculated from the disappearance of 1 X 10(-6) M 14C-5-HT from the medium, and LDH release and lipid peroxide formation were measured by spectrophotometric methods. The NO2 caused a significant increase in rhoDPH in both types of endothelial cells after 3 h and progressed with further exposure to NO2. Exposure to NO2 for 24 h, but not 3 or 12 h, significantly (p less than 0.05) reduced 5-HT uptake, increased (p less than 0.01) LDH release, and increased (p less than 0.05) lipid peroxide formation in both pulmonary artery and aortic endothelial cells. These results suggest that oxidant injury caused by NO changes the physical state of membrane lipids, impairs membrane function, and contributes to the biochemical and metabolic abnormalities in the cells." @default.
- W1710233745 created "2016-06-24" @default.
- W1710233745 creator A5040247825 @default.
- W1710233745 creator A5086158707 @default.
- W1710233745 date "1986-12-01" @default.
- W1710233745 modified "2023-09-23" @default.
- W1710233745 title "Nitrogen dioxide-induced changes in cell membrane fluidity and function." @default.
- W1710233745 cites W1242305241 @default.
- W1710233745 cites W1524923083 @default.
- W1710233745 cites W1529972356 @default.
- W1710233745 cites W1559900 @default.
- W1710233745 cites W1775749144 @default.
- W1710233745 cites W1976051504 @default.
- W1710233745 cites W1991655565 @default.
- W1710233745 cites W1997243937 @default.
- W1710233745 cites W2012231194 @default.
- W1710233745 cites W2021328933 @default.
- W1710233745 cites W2025357993 @default.
- W1710233745 cites W2063596745 @default.
- W1710233745 cites W2067280871 @default.
- W1710233745 cites W2073415444 @default.
- W1710233745 cites W2077237240 @default.
- W1710233745 cites W2078798516 @default.
- W1710233745 cites W2089114989 @default.
- W1710233745 cites W2090569361 @default.
- W1710233745 cites W2129995310 @default.
- W1710233745 cites W2292590484 @default.
- W1710233745 cites W2395675648 @default.
- W1710233745 cites W2410440997 @default.
- W1710233745 cites W2416964178 @default.
- W1710233745 doi "https://doi.org/10.1164/arrd.1986.134.5.1196" @default.
- W1710233745 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3789519" @default.
- W1710233745 hasPublicationYear "1986" @default.
- W1710233745 type Work @default.
- W1710233745 sameAs 1710233745 @default.
- W1710233745 citedByCount "17" @default.
- W1710233745 countsByYear W17102337452020 @default.
- W1710233745 crossrefType "journal-article" @default.
- W1710233745 hasAuthorship W1710233745A5040247825 @default.
- W1710233745 hasAuthorship W1710233745A5086158707 @default.
- W1710233745 hasConcept C107565407 @default.
- W1710233745 hasConcept C122398194 @default.
- W1710233745 hasConcept C12554922 @default.
- W1710233745 hasConcept C178790620 @default.
- W1710233745 hasConcept C181199279 @default.
- W1710233745 hasConcept C185592680 @default.
- W1710233745 hasConcept C2776151105 @default.
- W1710233745 hasConcept C2777318727 @default.
- W1710233745 hasConcept C2778106830 @default.
- W1710233745 hasConcept C2780723490 @default.
- W1710233745 hasConcept C2780829032 @default.
- W1710233745 hasConcept C2993790521 @default.
- W1710233745 hasConcept C39944091 @default.
- W1710233745 hasConcept C41625074 @default.
- W1710233745 hasConcept C55493867 @default.
- W1710233745 hasConcept C86803240 @default.
- W1710233745 hasConceptScore W1710233745C107565407 @default.
- W1710233745 hasConceptScore W1710233745C122398194 @default.
- W1710233745 hasConceptScore W1710233745C12554922 @default.
- W1710233745 hasConceptScore W1710233745C178790620 @default.
- W1710233745 hasConceptScore W1710233745C181199279 @default.
- W1710233745 hasConceptScore W1710233745C185592680 @default.
- W1710233745 hasConceptScore W1710233745C2776151105 @default.
- W1710233745 hasConceptScore W1710233745C2777318727 @default.
- W1710233745 hasConceptScore W1710233745C2778106830 @default.
- W1710233745 hasConceptScore W1710233745C2780723490 @default.
- W1710233745 hasConceptScore W1710233745C2780829032 @default.
- W1710233745 hasConceptScore W1710233745C2993790521 @default.
- W1710233745 hasConceptScore W1710233745C39944091 @default.
- W1710233745 hasConceptScore W1710233745C41625074 @default.
- W1710233745 hasConceptScore W1710233745C55493867 @default.
- W1710233745 hasConceptScore W1710233745C86803240 @default.
- W1710233745 hasIssue "6" @default.
- W1710233745 hasLocation W17102337451 @default.
- W1710233745 hasOpenAccess W1710233745 @default.
- W1710233745 hasPrimaryLocation W17102337451 @default.
- W1710233745 hasRelatedWork W1877370551 @default.
- W1710233745 hasRelatedWork W2052531890 @default.
- W1710233745 hasRelatedWork W2066171665 @default.
- W1710233745 hasRelatedWork W2075728727 @default.
- W1710233745 hasRelatedWork W2121971847 @default.
- W1710233745 hasRelatedWork W2165035054 @default.
- W1710233745 hasRelatedWork W2277990525 @default.
- W1710233745 hasRelatedWork W2921583591 @default.
- W1710233745 hasRelatedWork W3200594864 @default.
- W1710233745 hasRelatedWork W3214445124 @default.
- W1710233745 hasVolume "134" @default.
- W1710233745 isParatext "false" @default.
- W1710233745 isRetracted "false" @default.
- W1710233745 magId "1710233745" @default.
- W1710233745 workType "article" @default.