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- W171092145 abstract "Synaptosomes were prepared from the cerebral cortex, striatum, cerebellum, brain stem, and hippocampus of rats treated for five months on ethanol containing or sucrose-control liquid diets. Membrane properties were investigated using the fluorescence polarization of diphenylhexatriene. Fluorescence polarization of synaptosomal membranes was similar among all the control brain regions studied except the cerebral cortex which had significantly lower polarization values. Ethanol (30-500 mM) in vitro decreased polarization in all control synaptosomes. Cerebellar synaptosomal membranes and cerebral cortical membranes showed the greatest change whereas striatal showed much smaller decreases in polarization. Synaptosomal membranes prepared from rats treated with ethanol for 5 months had polarization values similar to control in the absence of ethanol. However, in the presence of ethanol brain stem synaptosomal membrane polarization was decreased 146 and 47 (p < 0.05) units in membranes from control and ethanol treated rats, respectively. Synaptosomal preparations from cerebellum and brain stem synaptosomes showed the greatest membrane tolerance with striatum having an intermediate tolerance. Hippocampal and cerebral cortical synaptosomes from control and ethanol treated rats showed no tolerance to the actions of ethanol." @default.
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- W171092145 date "1986-03-01" @default.
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- W171092145 title "Variations in brain region synaptosomal membrane organization, sensitivity, and tolerance to ethanol" @default.
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