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- W171205491 abstract "We developed a combined analysis of isolated cell dynamics based on both the extraction of relevant data obtained from time-lapse video-sequence analysis, such as velocities, periodic components, and preferential morphologies, and the simulation of cells’ dynamical behavior from theoretical models. Two methods have been alternatively used for analyzing the videomicroscopy sequences. The first one is based on an optical flow approach and relies on the conservation of light intensity in order to estimate the parameters of an affine motion model of cell deformations. A refined analysis of localized cell movements such as lamellipodial extension and retraction has also been undertaken using a more classical segmentation of cell outlines. Various tools have been subsequently used to analyze the corresponding time-amplitude curves and contours maps, as well as time variation of the motion model eigenvalues, including Fourier analysis. In association with this quantification of cell shape dynamics, we consider a continuous cytomechanical model of cell deformations. The model relies on the description of mechanical and chemical interactions among the cytoplasm, the actin cytoskeleton, and the cell membrane. Different patterns of cell morphological changes have been simulated, considering different values of the model parameters. Specific illustration of these combined approaches is proposed for the study of L929 murin fibroblast deformations." @default.
- W171205491 created "2016-06-24" @default.
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- W171205491 date "2003-01-01" @default.
- W171205491 modified "2023-10-16" @default.
- W171205491 title "Analysis of Cell Motility Combining Cytomechanical Model Simulations and an Optical Flow Method" @default.
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- W171205491 doi "https://doi.org/10.1007/978-3-0348-8043-5_9" @default.
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