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- W1714507077 abstract "The molecular mechanisms involved in cell adhesion have prompted considerable interest over the years. The study of interactions between cells and matrices in vitro is aimed at shedding some light on the extremely complicated system of interactions that must occur in vivo. The microexudate carpet as evidenced by scanning electron microscope observations promotes cell attachment and spreading of the cells. Thus biochemical studies of the mechanism of this phenomenon are feasible utilizing a modification of a quantitative adhesion assay. The assay is started by adding [3H]leucine-labeled singlecell suspension to each well of the multiple-well dish. The dish is then placed in a reciprocal shaker water bath at 37 ° and 50 strokes per minute. Adhesion kinetics are measured by removing by aspiration the nonadherent cells from wells at various times. The rate of adhesion is expressed as percentage of cells adhered by comparing the radioactivity in each well with that of a 1-ml aliquot of the original cell suspension to which 0.5 ml of 1.5 N NH4OH is added. Data obtained in this system indicates that active metabolism is required for adhesion, as evidenced by the inhibition by KCN and iodoacetate combined." @default.
- W1714507077 created "2016-06-24" @default.
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- W1714507077 date "1982-01-01" @default.
- W1714507077 modified "2023-09-26" @default.
- W1714507077 title "[27] Biosynthetic matrices from cells in culture" @default.
- W1714507077 cites W2000956724 @default.
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- W1714507077 doi "https://doi.org/10.1016/0076-6879(82)82084-3" @default.
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