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- W1715015369 abstract "This unit describes protocols for evaluating the pluripotency of embryonic and induced pluripotent stem cells using a teratoma formation assay. Cells are prepared for injection and transplanted into immunodeficient mice at the gastrocnemius muscle, a site well suited for teratoma growth and surgical access. Teratomas that form from the cell transplants are explanted, fixed in paraformaldehyde, and embedded in paraffin. These preserved samples are sectioned, stained, and analyzed. Pluripotency of a cell line is confirmed by whether the teratoma contains tissues derived from each of the embryonic germ layers: endoderm, mesoderm, and ectoderm. Alternatively, explanted and fixed teratomas can be cryopreserved for immunohistochemistry, which allows for more detailed identification of specific tissue types present in the samples. © 2015 by John Wiley & Sons, Inc." @default.
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- W1715015369 date "2015-02-01" @default.
- W1715015369 modified "2023-10-14" @default.
- W1715015369 title "Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research" @default.
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- W1715015369 doi "https://doi.org/10.1002/9780470151808.sc04a08s32" @default.
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