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• W1716042016 abstract "To study the secondary structure of the enzyme NADPH: protochlorophyllide oxidoreductase (PCOR), a novel method of enzyme isolation was developed. The detergent isotridecyl poly-(ethylene glycol) ether (Genapol X-080) selectively solubilizes the enzyme from a prolamellar-body fraction isolated from wheat (Triticum aestivum L.). The solubilized fraction was further purified by ion-exchange chromatography. The isolated enzyme was studied by fluorescence spectroscopy at 77 K, and by CD spectroscopy. The fluorescence-emission spectra revealed that the binding properties of the substrate and co-substrate were preserved and that photo-reduction occurred. The CD spectra of PCOR were analysed for the relative amounts of the secondary structures, alpha-helix, beta-sheet, turn and random coil. The secondary structure composition was estimated to be 33% alpha-helix, 19% beta-sheet, 20% turn and 28% random coil. These values are in agreement with those predicted by the Predict Heidelberg Deutschland and self-optimized prediction method from alignments methods. The enzyme has some amino acid identity with other NADPH-binding enzymes containing the Rossmann fold. The Rossmann-fold fingerprint motif is localized in the N-terminal region and at the expected positions in the predicted secondary structure. It is suggested that PCOR is anchored to the interfacial region of the membrane by either a beta-sheet or an alpha-helical region containing tryptophan residues. A hydrophobic loop-region could also be involved in membrane anchoring." @default.
• W1716042016 created "2016-06-24" @default.
• W1716042016 creator A5038481686 @default.
• W1716042016 creator A5071180846 @default.
• W1716042016 creator A5080895485 @default.
• W1716042016 date "1996-07-15" @default.
• W1716042016 modified "2023-09-25" @default.
• W1716042016 title "Secondary structure of NADPH: protochlorophyllide oxidoreductase examined by circular dichroism and prediction methods" @default.
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• W1716042016 doi "https://doi.org/10.1042/bj3170549" @default.
• W1716042016 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1217521" @default.
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