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- W1716339826 abstract "This chapter describes protocols for extract preparation, and methods for assessing nuclear changes of apoptosis along with more direct assays for detecting active caspases. The chapter discusses the cell-free systems used for the study of nuclear and mitochondrial changes of apoptosis brought about, to identify caspases activated during apoptosis, to investigate mechanisms of caspase activation, and to identify caspase substrates. Besides, to identify proteins necessary for apoptosis, cell-free systems investigate how inhibitors of apoptosis function. All of the in vitro apoptosis assays exploit apoptotic changes that depend on caspase activity. Some of these assays use isolated nuclei as readout for apoptotic changes whereas others measure caspase activity more directly. Direct assays of caspase activity further divided in those using caspase substrates and those using caspase processing as an indicator of activation. The chapter also discusses assays using isolated nuclei followed by the preparation of nuclei." @default.
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- W1716339826 date "2001-01-01" @default.
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- W1716339826 title "Chapter 7 Cell-free systems to study apoptosis" @default.
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- W1716339826 doi "https://doi.org/10.1016/s0091-679x(01)66008-4" @default.
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