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- W171666907 abstract "The present study was undertaken (a) to purify the enterotoxin and skin permeability factor of Aeromonas hydrophila using acetone fractionation, DEAE Sephadex A50 chromatography, ammonium sulphate precipitation and CM cellulose chromatography and (b) to study the mode of action of the enterotoxin in the rabbit ileal loop model. The enterotoxic factor for rabbit gut loop and the skin permeability factor were obtained as two separate protein fractions. Each gave a single band on polyacrylamide gel electrophoresis. An inoculum of 4 μ of the A. hydrophila enterotoxin caused fluid accumulation in the range of 1-2 ml in rabbit ileal loops. The skin permeability, factor when injected in doses of 1.5 μg elicited a positive blueing response as well as induration in rabbit skin. There was a 3-fold increase in the level of total 3‘5’ adenosine monophosphate (cyclic AMP) per mg protein in epithelial cells of rabbit ileal loops treated with culture filtrates and crude enterotoxin preparations of A. hydrophila for 6-8 h as compared to the level in normal intestinal epithelial cells. By analogy with Vibrio cholerae enterotoxin and Escherichia coli heat-labile enterotoxin, this is indicative of cyclic AMP mediation in the enterotoxic activity of A, hydrophila enterotoxin in rabbit gut loops." @default.
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- W171666907 date "1980-01-01" @default.
- W171666907 modified "2023-09-23" @default.
- W171666907 title "PURIFICATION OF AEROMONAS HYDROPHILA ENTEROTOXIN AND ITS MODE OF ACTION IN EXPERIMENTAL MODEL" @default.
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- W171666907 doi "https://doi.org/10.1016/b978-0-08-024952-0.50034-x" @default.
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