Matches in SemOpenAlex for { <https://semopenalex.org/work/W1718664314> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1718664314 endingPage "3046" @default.
- W1718664314 startingPage "3036" @default.
- W1718664314 abstract "Summary Metagenomics is an emerging field for mining the bioresources for new biomolecules for potential application in biotechnology and biomedicine. In the present study, a novel acetylhydrolase (Est13) was detected during the function‐based screening of a metagenomic library established from the DNA extracted from the cellulose‐depleting microbial community set up with an earthworm cast. Analysis showed that Est13 exhibited some similarities with a human and parasite platelet‐activating factor acetylhydrolase (PAF‐AH) belonging to the SGNH hydrolase superfamily. Biochemical characterization of the purified recombinant enzyme using substrates common for hydrolases of this superfamily demonstrated that Est13 hydrolysed p ‐nitrophenyl acetate quite efficiently, with a k cat / K M value of 3209 mM −1 s −1 . The Est13 showed highest activity at pH 8.0 and 40°C, conditions in which it is relatively stable compared with known PAF‐AHs. In vitro functional analysis of the platelet‐activating factor hydrolysis showed a dose‐ and time‐dependent inhibition of platelet aggregation in the range of 2–4 µM, making this enzyme a potential candidate for biomedical applications." @default.
- W1718664314 created "2016-06-24" @default.
- W1718664314 creator A5019913842 @default.
- W1718664314 creator A5036913314 @default.
- W1718664314 creator A5048477716 @default.
- W1718664314 creator A5054640028 @default.
- W1718664314 creator A5055431028 @default.
- W1718664314 creator A5058850197 @default.
- W1718664314 creator A5091772326 @default.
- W1718664314 date "2011-09-16" @default.
- W1718664314 modified "2023-10-16" @default.
- W1718664314 title "A novel platelet-activating factor acetylhydrolase discovered in a metagenome from the earthworm-associated microbial community" @default.
- W1718664314 cites W1964209287 @default.
- W1718664314 cites W1967901572 @default.
- W1718664314 cites W1977200885 @default.
- W1718664314 cites W1979640591 @default.
- W1718664314 cites W1980324165 @default.
- W1718664314 cites W1981264254 @default.
- W1718664314 cites W1985629904 @default.
- W1718664314 cites W1987581698 @default.
- W1718664314 cites W1991997954 @default.
- W1718664314 cites W1999501011 @default.
- W1718664314 cites W2009560933 @default.
- W1718664314 cites W2011415471 @default.
- W1718664314 cites W2027752637 @default.
- W1718664314 cites W2038057037 @default.
- W1718664314 cites W2042770508 @default.
- W1718664314 cites W2053930789 @default.
- W1718664314 cites W2058786689 @default.
- W1718664314 cites W2062887537 @default.
- W1718664314 cites W2066436790 @default.
- W1718664314 cites W2087063713 @default.
- W1718664314 cites W2093529460 @default.
- W1718664314 cites W2106882534 @default.
- W1718664314 cites W2117985613 @default.
- W1718664314 cites W2122504057 @default.
- W1718664314 cites W2126990958 @default.
- W1718664314 cites W2145880012 @default.
- W1718664314 cites W2158714788 @default.
- W1718664314 cites W2160053034 @default.
- W1718664314 cites W2407846868 @default.
- W1718664314 cites W4229620302 @default.
- W1718664314 cites W4293247451 @default.
- W1718664314 doi "https://doi.org/10.1111/j.1462-2920.2011.02581.x" @default.
- W1718664314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21923856" @default.
- W1718664314 hasPublicationYear "2011" @default.
- W1718664314 type Work @default.
- W1718664314 sameAs 1718664314 @default.
- W1718664314 citedByCount "9" @default.
- W1718664314 countsByYear W17186643142012 @default.
- W1718664314 countsByYear W17186643142013 @default.
- W1718664314 countsByYear W17186643142014 @default.
- W1718664314 countsByYear W17186643142015 @default.
- W1718664314 countsByYear W17186643142016 @default.
- W1718664314 countsByYear W17186643142018 @default.
- W1718664314 countsByYear W17186643142019 @default.
- W1718664314 crossrefType "journal-article" @default.
- W1718664314 hasAuthorship W1718664314A5019913842 @default.
- W1718664314 hasAuthorship W1718664314A5036913314 @default.
- W1718664314 hasAuthorship W1718664314A5048477716 @default.
- W1718664314 hasAuthorship W1718664314A5054640028 @default.
- W1718664314 hasAuthorship W1718664314A5055431028 @default.
- W1718664314 hasAuthorship W1718664314A5058850197 @default.
- W1718664314 hasAuthorship W1718664314A5091772326 @default.
- W1718664314 hasConcept C104317684 @default.
- W1718664314 hasConcept C150903083 @default.
- W1718664314 hasConcept C15151743 @default.
- W1718664314 hasConcept C181199279 @default.
- W1718664314 hasConcept C2780120296 @default.
- W1718664314 hasConcept C55493867 @default.
- W1718664314 hasConcept C86803240 @default.
- W1718664314 hasConceptScore W1718664314C104317684 @default.
- W1718664314 hasConceptScore W1718664314C150903083 @default.
- W1718664314 hasConceptScore W1718664314C15151743 @default.
- W1718664314 hasConceptScore W1718664314C181199279 @default.
- W1718664314 hasConceptScore W1718664314C2780120296 @default.
- W1718664314 hasConceptScore W1718664314C55493867 @default.
- W1718664314 hasConceptScore W1718664314C86803240 @default.
- W1718664314 hasIssue "11" @default.
- W1718664314 hasLocation W17186643141 @default.
- W1718664314 hasLocation W17186643142 @default.
- W1718664314 hasOpenAccess W1718664314 @default.
- W1718664314 hasPrimaryLocation W17186643141 @default.
- W1718664314 hasRelatedWork W1995816032 @default.
- W1718664314 hasRelatedWork W2359440920 @default.
- W1718664314 hasRelatedWork W2889550857 @default.
- W1718664314 hasRelatedWork W2968048207 @default.
- W1718664314 hasRelatedWork W2968354375 @default.
- W1718664314 hasRelatedWork W3013372948 @default.
- W1718664314 hasRelatedWork W3135997498 @default.
- W1718664314 hasRelatedWork W3198631238 @default.
- W1718664314 hasRelatedWork W4242417330 @default.
- W1718664314 hasRelatedWork W638577851 @default.
- W1718664314 hasVolume "13" @default.
- W1718664314 isParatext "false" @default.
- W1718664314 isRetracted "false" @default.
- W1718664314 magId "1718664314" @default.
- W1718664314 workType "article" @default.