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• W171882232 abstract "Plants use light energy, carbon dioxide and water to produce sugars and other carbohydrates, which serve as stored energy reserves and as building blocks for biosynthetic reactions. Supply of light is variable and plants have evolved means to adjust their growth and development accordingly. An increasing body of evidence suggests that the basic mechanisms for sensing and signaling energy availability in eukaryotes are evolutionary conserved and thus shared between plants, animals and fungi. I have used different experimental approaches that take advantage of findings from other eukaryotes in studying carbon and energy metabolism in plants. In the first part, I developed a novel screening procedure in yeast aimed at isolating cDNAs from other organisms encoding proteins with a possible function in sugar sensing or signaling. The feasibility of the method was confirmed by the cloning of a cDNA from Arabidopsis thaliana encoding a new F-box protein named AtGrh1, which is related to the yeast Grr1 protein that is involved in glucose repression. In the second part of the study, plant homologues of key components in the yeast glucose repression pathway were cloned and characterized in the moss Physcomitrella patens, in which gene function can be studied by gene targeting. We first cloned PpHXK1 which was shown to encode a chloroplast localized hexokinase representing a previously overlooked class of plant hexokinases with an N-terminal chloroplast transit peptide. Significantly, PpHxk1 is the major hexokinase in Physcomitrella, accounting for 80% of the glucose phosphorylating activity. A knockout mutant deleted for PpHXK1 exhibits a complex phenotype affecting growth, development and sensitivities to plant hormones. I also cloned and characterized two closely related Physcomitrella genes, PpSNF1a and PpSNF1b, encoding type 1 Snf1-related kinases. A double knockout mutant for these genes was viable even though it lacks detectable Snf1-like kinase activity. The mutant suffers from pleiotropic phenotypes which may reflect a constitutive high energy growth mode. Significantly, the double mutant requires constant high light and is therefore unable to grow in a normal day/night light cycle. These findings are consistent with the proposed role of the Snf1-related kinases as energy gauges which are needed to recognize and respond to low energy conditions." @default.
• W171882232 created "2016-06-24" @default.
• W171882232 creator A5089247893 @default.
• W171882232 date "2003-11-01" @default.
• W171882232 modified "2023-09-27" @default.
• W171882232 title "Studies of molecular mechanisms integrating carbon metabolism and growth in plants" @default.
• W171882232 cites W1492180865 @default.
• W171882232 cites W1559442263 @default.
• W171882232 cites W1564173783 @default.
• W171882232 cites W1575732080 @default.
• W171882232 cites W1597997906 @default.
• W171882232 cites W1607014413 @default.
• W171882232 cites W1663808526 @default.
• W171882232 cites W1755773572 @default.
• W171882232 cites W1763161027 @default.
• W171882232 cites W1821968258 @default.
• W171882232 cites W1825003750 @default.
• W171882232 cites W1849926859 @default.
• W171882232 cites W1856461783 @default.
• W171882232 cites W1875023925 @default.
• W171882232 cites W187623135 @default.
• W171882232 cites W1906849814 @default.
• W171882232 cites W1955808217 @default.
• W171882232 cites W1958996383 @default.
• W171882232 cites W1965253996 @default.
• W171882232 cites W1965254867 @default.
• W171882232 cites W1965717790 @default.
• W171882232 cites W1966522270 @default.
• W171882232 cites W1973816983 @default.
• W171882232 cites W1975012490 @default.
• W171882232 cites W1977880141 @default.
• W171882232 cites W1978739303 @default.
• W171882232 cites W1985233399 @default.
• W171882232 cites W1985910275 @default.
• W171882232 cites W1988346998 @default.
• W171882232 cites W1988608160 @default.
• W171882232 cites W1995169033 @default.
• W171882232 cites W1995683339 @default.
• W171882232 cites W1996119276 @default.
• W171882232 cites W2000047924 @default.
• W171882232 cites W2000168109 @default.
• W171882232 cites W2000659699 @default.
• W171882232 cites W2003357102 @default.
• W171882232 cites W2003698297 @default.
• W171882232 cites W2003979548 @default.
• W171882232 cites W2004966136 @default.
• W171882232 cites W2005812143 @default.
• W171882232 cites W2011187494 @default.
• W171882232 cites W2012260529 @default.
• W171882232 cites W2015063854 @default.
• W171882232 cites W2015890225 @default.
• W171882232 cites W2015924572 @default.
• W171882232 cites W2017260714 @default.
• W171882232 cites W2019140968 @default.
• W171882232 cites W2021336594 @default.
• W171882232 cites W2021470522 @default.
• W171882232 cites W2028670909 @default.
• W171882232 cites W2030847509 @default.
• W171882232 cites W2047879424 @default.
• W171882232 cites W2047967481 @default.
• W171882232 cites W2050028641 @default.
• W171882232 cites W2052571494 @default.
• W171882232 cites W2057501778 @default.
• W171882232 cites W2059028222 @default.
• W171882232 cites W2059825898 @default.
• W171882232 cites W2061840340 @default.
• W171882232 cites W2069010013 @default.
• W171882232 cites W2075531794 @default.
• W171882232 cites W2075746053 @default.
• W171882232 cites W2076143315 @default.
• W171882232 cites W2076913878 @default.
• W171882232 cites W2077248514 @default.
• W171882232 cites W2077821403 @default.
• W171882232 cites W2078223676 @default.
• W171882232 cites W2084391532 @default.
• W171882232 cites W2085071291 @default.
• W171882232 cites W2086269065 @default.
• W171882232 cites W2086488294 @default.
• W171882232 cites W2086968057 @default.
• W171882232 cites W2088542735 @default.
• W171882232 cites W2088839196 @default.
• W171882232 cites W2091567823 @default.
• W171882232 cites W2092402636 @default.
• W171882232 cites W2094160630 @default.
• W171882232 cites W2096580557 @default.
• W171882232 cites W2097382368 @default.
• W171882232 cites W2099164892 @default.
• W171882232 cites W2099685366 @default.
• W171882232 cites W2099715003 @default.
• W171882232 cites W2100444609 @default.
• W171882232 cites W2102204014 @default.
• W171882232 cites W2106284532 @default.
• W171882232 cites W2107775693 @default.
• W171882232 cites W2107800719 @default.
• W171882232 cites W2108023916 @default.
• W171882232 cites W2111278461 @default.
• W171882232 cites W2112556771 @default.
• W171882232 cites W2115168068 @default.
• W171882232 cites W2117544585 @default.
• W171882232 cites W2117548944 @default.

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