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- W1720734481 abstract "Demineralization of teeth with their adherent bacterial flora is a prerequisite for the localization of selected species in sections of undisturbed bacterial deposits by immunoelectron microscopy. The effect of EDTA dernineralization on the immunological labeling of Gram-positive and Gram-negative oral bacteria was tested on strains of Actinomycesviscosus, A. israelii, Eikertella corrodens, Bacieroides meloninogenicus. Capnocytophaga sputigena and Wolinella recta. After 3 months in EDTA, cells prefixed in paraformaldehyde-picric acid were washed and incubated with homologous rabbit antiscrum followed by goat anti-rabbit IgG to enhance the immunocoating reaction. Samples were then processed for transmission electron microscopy. Control specimens included (1) demineralized cells incubated with pre-immune rabbit sera instead of homologous antisera (2) cells stored for 3 months in PBS instead of EDTA (3) fresh cells processed for immunocoating immediately after prefixation and washing. No detectable immunocoating layer was found on the cells of the control group incubated with pre-immune rabbitserum, EDTA treated cells demonstrated an immunocoating layer of similar density and thickness to that of cells stored in PBS, or fresh cells. The results indicate that immunoelectron microscopic localization of bacterial cells may be feasible in ullrathin sections of demineralized dental specimens." @default.
- W1720734481 created "2016-06-24" @default.
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- W1720734481 date "1981-06-01" @default.
- W1720734481 modified "2023-09-26" @default.
- W1720734481 title "Immunological labeling of oral bacteria after demineralization." @default.
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- W1720734481 doi "https://doi.org/10.1111/j.1600-0765.1981.tb00975.x" @default.
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