Matches in SemOpenAlex for { <https://semopenalex.org/work/W1723169767> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1723169767 endingPage "95" @default.
- W1723169767 startingPage "90" @default.
- W1723169767 abstract "Problem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many binary vectors are quite large in size and contain few useful restriction sites making their in vitro manipulation technically challenging. Approach: A small (7698 bp) and flexible binary vector named pGPro4 was constructed to possess unique features favorable for promoter analysis in dicot plants. A nopaline synthase (nos) promoter was used to control the expression of the selectable marker of pGPro4 to prevent the problem of interference with the neighboring promoter-reporter fusion. In pGPro4, the nos promoter and hygromycin phosphotransferase II (hptII) sequences are flanked by loxP sites, which allow for Cre recombinase-mediated removal when hygromycin resistance is no longer desired. pGPro4 also contains a bifunctional β-glucuronidase-enhanced Green Fluorescent Protein (gusA-eGFP) reporter gene that provides visual detection of reporter gene expression using either fluorescence in live cells or histochemical detection of β-glucuronidase activity. Results and Conclusion: To demonstrate the usefulness of the pGPro4 vector, a CaMV35S promoter was fused to gusA-eGFP and the resulting plasmid, pGPro4-35S, was used to transform Lesquerella fendleri. Primary shoots were generated from explants at an expected frequency of 10-27.5%, indicating that the nos promoter drove sufficient hptII expression to generate hygromycin resistant plants. Six independent transgenic L. fendleri lines were grown to maturity and generated T1 seeds. The bifunctionality of the gusA-eGFP reporter gene was verified by detecting both green fluorescence and β-glucuronidase activity in multiple T1 L. fendleri seedlings from 5 of the 6 the independent transgenic lines." @default.
- W1723169767 created "2016-06-24" @default.
- W1723169767 creator A5025974478 @default.
- W1723169767 date "2011-10-01" @default.
- W1723169767 modified "2023-09-25" @default.
- W1723169767 title "Transformation of <i>Lesquerella Fendleri</i> with the New Binary Vector pGPro4-35S" @default.
- W1723169767 cites W1539503448 @default.
- W1723169767 cites W1546321334 @default.
- W1723169767 cites W1585483179 @default.
- W1723169767 cites W1980102367 @default.
- W1723169767 cites W1981057628 @default.
- W1723169767 cites W1988092647 @default.
- W1723169767 cites W2002171861 @default.
- W1723169767 cites W2025078829 @default.
- W1723169767 cites W2064184262 @default.
- W1723169767 cites W2071312048 @default.
- W1723169767 cites W2076434298 @default.
- W1723169767 cites W2089184691 @default.
- W1723169767 cites W2128070038 @default.
- W1723169767 cites W2128400180 @default.
- W1723169767 cites W2139751264 @default.
- W1723169767 cites W2152914706 @default.
- W1723169767 cites W2158278323 @default.
- W1723169767 cites W618589720 @default.
- W1723169767 cites W1509592630 @default.
- W1723169767 doi "https://doi.org/10.3844/ojbsci.2011.90.95" @default.
- W1723169767 hasPublicationYear "2011" @default.
- W1723169767 type Work @default.
- W1723169767 sameAs 1723169767 @default.
- W1723169767 citedByCount "4" @default.
- W1723169767 countsByYear W17231697672012 @default.
- W1723169767 countsByYear W17231697672014 @default.
- W1723169767 countsByYear W17231697672016 @default.
- W1723169767 crossrefType "journal-article" @default.
- W1723169767 hasAuthorship W1723169767A5025974478 @default.
- W1723169767 hasBestOaLocation W17231697671 @default.
- W1723169767 hasConcept C100012643 @default.
- W1723169767 hasConcept C101762097 @default.
- W1723169767 hasConcept C102230213 @default.
- W1723169767 hasConcept C104317684 @default.
- W1723169767 hasConcept C111936080 @default.
- W1723169767 hasConcept C142613039 @default.
- W1723169767 hasConcept C150194340 @default.
- W1723169767 hasConcept C153911025 @default.
- W1723169767 hasConcept C161733203 @default.
- W1723169767 hasConcept C204241405 @default.
- W1723169767 hasConcept C22744801 @default.
- W1723169767 hasConcept C2779260011 @default.
- W1723169767 hasConcept C33161422 @default.
- W1723169767 hasConcept C40767141 @default.
- W1723169767 hasConcept C42411736 @default.
- W1723169767 hasConcept C54355233 @default.
- W1723169767 hasConcept C86803240 @default.
- W1723169767 hasConcept C92087593 @default.
- W1723169767 hasConceptScore W1723169767C100012643 @default.
- W1723169767 hasConceptScore W1723169767C101762097 @default.
- W1723169767 hasConceptScore W1723169767C102230213 @default.
- W1723169767 hasConceptScore W1723169767C104317684 @default.
- W1723169767 hasConceptScore W1723169767C111936080 @default.
- W1723169767 hasConceptScore W1723169767C142613039 @default.
- W1723169767 hasConceptScore W1723169767C150194340 @default.
- W1723169767 hasConceptScore W1723169767C153911025 @default.
- W1723169767 hasConceptScore W1723169767C161733203 @default.
- W1723169767 hasConceptScore W1723169767C204241405 @default.
- W1723169767 hasConceptScore W1723169767C22744801 @default.
- W1723169767 hasConceptScore W1723169767C2779260011 @default.
- W1723169767 hasConceptScore W1723169767C33161422 @default.
- W1723169767 hasConceptScore W1723169767C40767141 @default.
- W1723169767 hasConceptScore W1723169767C42411736 @default.
- W1723169767 hasConceptScore W1723169767C54355233 @default.
- W1723169767 hasConceptScore W1723169767C86803240 @default.
- W1723169767 hasConceptScore W1723169767C92087593 @default.
- W1723169767 hasIssue "3" @default.
- W1723169767 hasLocation W17231697671 @default.
- W1723169767 hasOpenAccess W1723169767 @default.
- W1723169767 hasPrimaryLocation W17231697671 @default.
- W1723169767 hasRelatedWork W1723169767 @default.
- W1723169767 hasRelatedWork W1988092647 @default.
- W1723169767 hasRelatedWork W2015754904 @default.
- W1723169767 hasRelatedWork W2039159996 @default.
- W1723169767 hasRelatedWork W2076884288 @default.
- W1723169767 hasRelatedWork W2092359053 @default.
- W1723169767 hasRelatedWork W2151560605 @default.
- W1723169767 hasRelatedWork W2730559070 @default.
- W1723169767 hasRelatedWork W2809426301 @default.
- W1723169767 hasRelatedWork W3013210851 @default.
- W1723169767 hasVolume "11" @default.
- W1723169767 isParatext "false" @default.
- W1723169767 isRetracted "false" @default.
- W1723169767 magId "1723169767" @default.
- W1723169767 workType "article" @default.