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- W1723341713 abstract "We isolated a highly transformable thermophile, Bacillus stearothermophilus SIC1, which exhibited the following features. The growth temperature ranged from 45 to 65°C in L broth. The maximum cell concentration in 2L broth (2% tryptone, 1% yeast extract, 0.5% NaCl, pH 7.2) was determined as an optical density at 660 nm of 7.8, and the generation time was 11 min at 60°C. Strain SIC1 was a prototroph and was transformed by the protoplast procedure not only with repB plasmids (high-copy-number plasmids such as pTB913 and pUB110) but also with repA plasmids (low-copy-number plasmids such as pTB53). Transformation efficiencies with repB and repA plasmids were about 2 × 10 6 to 5 × 10 6 and 5 × 10 4 transformants per μg of DNA, respectively. The transformant carrying plasmid pTB913Y/K could grow at 63°C in the presence of kanamycin. The regeneration frequency of protoplasts was 60%, and only 1 day was needed for regeneration at 55°C." @default.
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- W1723341713 date "1988-12-01" @default.
- W1723341713 modified "2023-09-23" @default.
- W1723341713 title "Useful Host-Vector Systems in Bacillus stearothermophilus" @default.
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- W1723341713 doi "https://doi.org/10.1128/aem.54.12.3162-3164.1988" @default.
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