FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1724980956> ?p ?o ?g. }

• W1724980956 endingPage "346" @default.
• W1724980956 startingPage "335" @default.
• W1724980956 abstract "The addition of pyruvate to the culture medium has been reported to improve the maintenance of P450-dependent enzyme expression in primary rat hepatocyte cultures. In this study, the effects of 30mM pyruvate on cell morphology, albumin secretion and glutathione S-transferase (GST) expression were investigated as a function of the time in culture. The effect of triiodothyronine (T3) exposure on GST expression was also measured in pyruvate-treated cultures. Transmission electron microscopy showed that untreated hepatocytes deteriorated after culture for 7 days, whereas the morphology of the pyruvate-treated cells was similar to that observed in intact liver tissue. The albumin secretion rate was significantly higher in rat hepatocytes exposed to pyruvate than in control cells. In the presence of pyruvate, mu and alpha class GST activities were well maintained, whereas GST pi activity was increased over the entire culture period. HPLC analysis revealed that the complement of GST subunits present in hepatocytes is altered during culture with pyruvate: mu,class proteins remained relatively constant, whereas a decrease in the alpha class content was accompanied by a strong increase in GST subunit P1 (GSTP1). The induction of GSTP1 was confirmed at the mRNA level. In control cultures, pi class GST activity was increased, but total, mu, and alpha class GST activities continuously declined as a function of culture time and became undetectable beyond 7 days in culture. At the protein and mRNA levels, a much smaller increase in GSTP1 was observed than in the pyruvate cultures. When the pyruvate-treated cell cultures were exposed to T3, an inhibitory effect on GST activities and proteins was found. These results indicate that this simple culture model could be useful for studying the expression and regulation of GST." @default.
• W1724980956 created "2016-06-24" @default.
• W1724980956 creator A5008668373 @default.
• W1724980956 creator A5022384436 @default.
• W1724980956 creator A5036015621 @default.
• W1724980956 creator A5042631543 @default.
• W1724980956 creator A5056011636 @default.
• W1724980956 creator A5077745548 @default.
• W1724980956 date "2001-05-01" @default.
• W1724980956 modified "2023-10-14" @default.
• W1724980956 title "Pyruvate-induced Long-term Maintenance of Glutathione S-Transferase in Rat Hepatocyte Cultures" @default.
• W1724980956 cites W1536916609 @default.
• W1724980956 cites W1536923222 @default.
• W1724980956 cites W1581414347 @default.
• W1724980956 cites W1854748115 @default.
• W1724980956 cites W1964329108 @default.
• W1724980956 cites W1967443513 @default.
• W1724980956 cites W1967987830 @default.
• W1724980956 cites W1968416349 @default.
• W1724980956 cites W1971924987 @default.
• W1724980956 cites W1974394774 @default.
• W1724980956 cites W1976204029 @default.
• W1724980956 cites W1981871881 @default.
• W1724980956 cites W1983796700 @default.
• W1724980956 cites W1999102410 @default.
• W1724980956 cites W2005091054 @default.
• W1724980956 cites W2011853969 @default.
• W1724980956 cites W2019454655 @default.
• W1724980956 cites W2021816068 @default.
• W1724980956 cites W2044574677 @default.
• W1724980956 cites W2062652813 @default.
• W1724980956 cites W2067194279 @default.
• W1724980956 cites W2074385257 @default.
• W1724980956 cites W2076013350 @default.
• W1724980956 cites W2079446105 @default.
• W1724980956 cites W2082151507 @default.
• W1724980956 cites W2088972842 @default.
• W1724980956 cites W2109922742 @default.
• W1724980956 cites W2117283508 @default.
• W1724980956 cites W2147391967 @default.
• W1724980956 cites W2336688239 @default.
• W1724980956 cites W2344678176 @default.
• W1724980956 cites W294744944 @default.
• W1724980956 cites W3025790143 @default.
• W1724980956 cites W4237213963 @default.
• W1724980956 cites W4294216491 @default.
• W1724980956 cites W4384902948 @default.
• W1724980956 doi "https://doi.org/10.1177/026119290102900324" @default.
• W1724980956 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11387028" @default.
• W1724980956 hasPublicationYear "2001" @default.
• W1724980956 type Work @default.
• W1724980956 sameAs 1724980956 @default.
• W1724980956 citedByCount "4" @default.
• W1724980956 countsByYear W17249809562016 @default.
• W1724980956 countsByYear W17249809562023 @default.
• W1724980956 crossrefType "journal-article" @default.
• W1724980956 hasAuthorship W1724980956A5008668373 @default.
• W1724980956 hasAuthorship W1724980956A5022384436 @default.
• W1724980956 hasAuthorship W1724980956A5036015621 @default.
• W1724980956 hasAuthorship W1724980956A5042631543 @default.
• W1724980956 hasAuthorship W1724980956A5056011636 @default.
• W1724980956 hasAuthorship W1724980956A5077745548 @default.
• W1724980956 hasBestOaLocation W17249809561 @default.
• W1724980956 hasConcept C126322002 @default.
• W1724980956 hasConcept C134018914 @default.
• W1724980956 hasConcept C153911025 @default.
• W1724980956 hasConcept C181199279 @default.
• W1724980956 hasConcept C202751555 @default.
• W1724980956 hasConcept C2776200302 @default.
• W1724980956 hasConcept C2776907368 @default.
• W1724980956 hasConcept C538909803 @default.
• W1724980956 hasConcept C54355233 @default.
• W1724980956 hasConcept C55493867 @default.
• W1724980956 hasConcept C71924100 @default.
• W1724980956 hasConcept C81885089 @default.
• W1724980956 hasConcept C86803240 @default.
• W1724980956 hasConceptScore W1724980956C126322002 @default.
• W1724980956 hasConceptScore W1724980956C134018914 @default.
• W1724980956 hasConceptScore W1724980956C153911025 @default.
• W1724980956 hasConceptScore W1724980956C181199279 @default.
• W1724980956 hasConceptScore W1724980956C202751555 @default.
• W1724980956 hasConceptScore W1724980956C2776200302 @default.
• W1724980956 hasConceptScore W1724980956C2776907368 @default.
• W1724980956 hasConceptScore W1724980956C538909803 @default.
• W1724980956 hasConceptScore W1724980956C54355233 @default.
• W1724980956 hasConceptScore W1724980956C55493867 @default.
• W1724980956 hasConceptScore W1724980956C71924100 @default.
• W1724980956 hasConceptScore W1724980956C81885089 @default.
• W1724980956 hasConceptScore W1724980956C86803240 @default.
• W1724980956 hasIssue "3" @default.
• W1724980956 hasLocation W17249809561 @default.
• W1724980956 hasLocation W17249809562 @default.
• W1724980956 hasOpenAccess W1724980956 @default.
• W1724980956 hasPrimaryLocation W17249809561 @default.
• W1724980956 hasRelatedWork W1485204759 @default.
• W1724980956 hasRelatedWork W173958657 @default.
• W1724980956 hasRelatedWork W1974921848 @default.
• W1724980956 hasRelatedWork W1998649742 @default.

• next