FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W172569458> ?p ?o ?g. }

• W172569458 endingPage "508" @default.
• W172569458 startingPage "491" @default.
• W172569458 abstract "The Lipase Engineering Database is a WWW-accessible resource on sequence–structure–function relationships of microbial lipases. Sequences of 92 microbial lipases and homologous serine hydrolases were assigned to 32 homologous families and 15 superfamilies. Multisequence alignments of all homologous families and superfamilies are provided. Functionally relevant amino acids are annotated. The catalytic serine is part of the conserved nucleophilic elbow and was identified in all sequences by its conserved signature GxSxG. The complete catalytic machinery (catalytic triad and two oxyanion hole residues) could be annotated in 91% of LED sequence entries. Published mutants and their properties are provided. The X-ray structures of 22 lipases were superposed and consistently annotated. Sequence and structure data were applied to study the role of the first oxyanion hole residues. Although the backbone amides contribute to the oxyanion hole rather than side chains, the residues are well conserved. Two sequence signatures including the first oxyanion hole residue were identified: GX and GGGX. In the GX type, the position of the first oxyanion hole residue X is stabilized by one or several anchor residues. If X is hydrophilic, it is hydrogen bonded to hydrophilic anchor residues, while hydrophobic oxyanion hole residues bind to hydrophobic pockets. The GGGX type includes short chain length specific lipases and carboxylesterases. The first oxyanion hole residue G is stabilized by interaction of the dipeptide GX with the side chain of the second oxyanion hole residues, which is a conserved alanine as C-terminal neighbour of the catalytic serine. Thus, short chain specific lipases and carboxylesterase can be identified by combining the signatures GGGX and GxSAG. Consistently annotated aligned sequences and superimposed structures of microbial lipases help to understand the functional role of individual amino acids and thus the LED is a useful tool for protein engineering. The Lipase Engineering Database is available at http://www.led.uni-stuttgart.de/." @default.
• W172569458 created "2016-06-24" @default.
• W172569458 creator A5006304159 @default.
• W172569458 creator A5026647644 @default.
• W172569458 creator A5027651817 @default.
• W172569458 creator A5067406221 @default.
• W172569458 creator A5073187609 @default.
• W172569458 date "2000-10-01" @default.
• W172569458 modified "2023-10-18" @default.
• W172569458 title "Lipase engineering database" @default.
• W172569458 cites W1508596864 @default.
• W172569458 cites W1509844421 @default.
• W172569458 cites W1730348256 @default.
• W172569458 cites W1781120710 @default.
• W172569458 cites W1963722789 @default.
• W172569458 cites W1966041739 @default.
• W172569458 cites W2003589493 @default.
• W172569458 cites W2008708467 @default.
• W172569458 cites W2009570597 @default.
• W172569458 cites W2018516269 @default.
• W172569458 cites W2020088601 @default.
• W172569458 cites W2023790301 @default.
• W172569458 cites W2048069826 @default.
• W172569458 cites W2061498308 @default.
• W172569458 cites W2061833373 @default.
• W172569458 cites W2064357683 @default.
• W172569458 cites W2064910354 @default.
• W172569458 cites W2067431380 @default.
• W172569458 cites W2067819039 @default.
• W172569458 cites W2072187094 @default.
• W172569458 cites W2074511040 @default.
• W172569458 cites W2081186248 @default.
• W172569458 cites W2085814338 @default.
• W172569458 cites W2085958374 @default.
• W172569458 cites W2086503758 @default.
• W172569458 cites W2094243160 @default.
• W172569458 cites W2097382368 @default.
• W172569458 cites W2100018338 @default.
• W172569458 cites W2104401146 @default.
• W172569458 cites W2108067237 @default.
• W172569458 cites W2116499195 @default.
• W172569458 cites W2117077088 @default.
• W172569458 cites W2158714788 @default.
• W172569458 cites W2158888718 @default.
• W172569458 cites W2167012856 @default.
• W172569458 cites W2414307244 @default.
• W172569458 cites W2916193864 @default.
• W172569458 cites W4238935654 @default.
• W172569458 cites W4240620708 @default.
• W172569458 cites W4248441758 @default.
• W172569458 cites W4297092563 @default.
• W172569458 cites W4320581636 @default.
• W172569458 doi "https://doi.org/10.1016/s1381-1177(00)00092-8" @default.
• W172569458 hasPublicationYear "2000" @default.
• W172569458 type Work @default.
• W172569458 sameAs 172569458 @default.
• W172569458 citedByCount "159" @default.
• W172569458 countsByYear W1725694582012 @default.
• W172569458 countsByYear W1725694582013 @default.
• W172569458 countsByYear W1725694582014 @default.
• W172569458 countsByYear W1725694582015 @default.
• W172569458 countsByYear W1725694582016 @default.
• W172569458 countsByYear W1725694582017 @default.
• W172569458 countsByYear W1725694582018 @default.
• W172569458 countsByYear W1725694582019 @default.
• W172569458 countsByYear W1725694582020 @default.
• W172569458 countsByYear W1725694582021 @default.
• W172569458 countsByYear W1725694582022 @default.
• W172569458 countsByYear W1725694582023 @default.
• W172569458 crossrefType "journal-article" @default.
• W172569458 hasAuthorship W172569458A5006304159 @default.
• W172569458 hasAuthorship W172569458A5026647644 @default.
• W172569458 hasAuthorship W172569458A5027651817 @default.
• W172569458 hasAuthorship W172569458A5067406221 @default.
• W172569458 hasAuthorship W172569458A5073187609 @default.
• W172569458 hasConcept C145770059 @default.
• W172569458 hasConcept C161790260 @default.
• W172569458 hasConcept C179506582 @default.
• W172569458 hasConcept C181199279 @default.
• W172569458 hasConcept C185592680 @default.
• W172569458 hasConcept C2776414213 @default.
• W172569458 hasConcept C2778934325 @default.
• W172569458 hasConcept C2779697368 @default.
• W172569458 hasConcept C2781338088 @default.
• W172569458 hasConcept C55493867 @default.
• W172569458 hasConcept C71240020 @default.
• W172569458 hasConceptScore W172569458C145770059 @default.
• W172569458 hasConceptScore W172569458C161790260 @default.
• W172569458 hasConceptScore W172569458C179506582 @default.
• W172569458 hasConceptScore W172569458C181199279 @default.
• W172569458 hasConceptScore W172569458C185592680 @default.
• W172569458 hasConceptScore W172569458C2776414213 @default.
• W172569458 hasConceptScore W172569458C2778934325 @default.
• W172569458 hasConceptScore W172569458C2779697368 @default.
• W172569458 hasConceptScore W172569458C2781338088 @default.
• W172569458 hasConceptScore W172569458C55493867 @default.
• W172569458 hasConceptScore W172569458C71240020 @default.
• W172569458 hasIssue "5" @default.

• next