Matches in SemOpenAlex for { <https://semopenalex.org/work/W1728203356> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1728203356 endingPage "342" @default.
- W1728203356 startingPage "320" @default.
- W1728203356 abstract "This chapter describes the immunotitration of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase. Extensive evidence supports the conclusion that HMG-CoA reductase [mevalonate:NADP+ oxidoreductase (CoA-acylating)] is the major regulatory enzyme in the biosynthesis of cholesterol. A technique for the study of rat liver HMG-CoA reductase that utilizes immunotitration of enzyme activity, with HMG-CoA reductase antiserum, is discussed in the chapter. The specific activity of the purified HMG-CoA reductase utilized as the antigen is approximately 500 nmol of mevalonate formed per minute per milligram protein. HMG-CoA reductase antiserum is evaluated, by immunodiffusion and by quantitative titration of both solubilized enzyme, as well as microsomal HMG-CoA reductase. Mevalonate formation is assayed, by a rapid chloroform extraction assay, which is equivalent in accuracy to thin-layer chromatography. The use of immunotitration of HMG-CoA reductase is aimed at providing quantitative estimates of regulation occurring, by changes in the enzyme concentration and by changes in the enzyme activity. It is found that cholestyramine-treated animals contained substantially more HMG-CoA reductase enzyme than untreated animals." @default.
- W1728203356 created "2016-06-24" @default.
- W1728203356 creator A5024577124 @default.
- W1728203356 creator A5055227492 @default.
- W1728203356 creator A5078248030 @default.
- W1728203356 date "1981-01-01" @default.
- W1728203356 modified "2023-10-15" @default.
- W1728203356 title "[21] Immunotitration of 3-hydroxy-3-methylglutaryl-CoA reductase" @default.
- W1728203356 cites W13351788 @default.
- W1728203356 cites W1489602901 @default.
- W1728203356 cites W1491129049 @default.
- W1728203356 cites W1500360994 @default.
- W1728203356 cites W1525888411 @default.
- W1728203356 cites W1557733310 @default.
- W1728203356 cites W1581328433 @default.
- W1728203356 cites W1581906851 @default.
- W1728203356 cites W1970938331 @default.
- W1728203356 cites W1975445796 @default.
- W1728203356 cites W1986481218 @default.
- W1728203356 cites W2003756649 @default.
- W1728203356 cites W2010388669 @default.
- W1728203356 cites W2018469567 @default.
- W1728203356 cites W2038727682 @default.
- W1728203356 cites W2044828194 @default.
- W1728203356 cites W2057158122 @default.
- W1728203356 cites W2068956083 @default.
- W1728203356 cites W2070442185 @default.
- W1728203356 cites W2070538868 @default.
- W1728203356 cites W2083625204 @default.
- W1728203356 cites W2086919263 @default.
- W1728203356 cites W2105877460 @default.
- W1728203356 cites W2165313725 @default.
- W1728203356 cites W2182500437 @default.
- W1728203356 cites W2187614775 @default.
- W1728203356 cites W2346140869 @default.
- W1728203356 cites W2396263213 @default.
- W1728203356 cites W2416843073 @default.
- W1728203356 cites W314515482 @default.
- W1728203356 cites W4256377460 @default.
- W1728203356 cites W52919052 @default.
- W1728203356 doi "https://doi.org/10.1016/0076-6879(81)74023-0" @default.
- W1728203356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6798367" @default.
- W1728203356 hasPublicationYear "1981" @default.
- W1728203356 type Work @default.
- W1728203356 sameAs 1728203356 @default.
- W1728203356 citedByCount "5" @default.
- W1728203356 crossrefType "book-chapter" @default.
- W1728203356 hasAuthorship W1728203356A5024577124 @default.
- W1728203356 hasAuthorship W1728203356A5055227492 @default.
- W1728203356 hasAuthorship W1728203356A5078248030 @default.
- W1728203356 hasConcept C104788683 @default.
- W1728203356 hasConcept C104950815 @default.
- W1728203356 hasConcept C134651460 @default.
- W1728203356 hasConcept C147483822 @default.
- W1728203356 hasConcept C153911025 @default.
- W1728203356 hasConcept C181199279 @default.
- W1728203356 hasConcept C185592680 @default.
- W1728203356 hasConcept C2777320049 @default.
- W1728203356 hasConcept C2778163477 @default.
- W1728203356 hasConcept C2778697171 @default.
- W1728203356 hasConcept C2779123261 @default.
- W1728203356 hasConcept C54355233 @default.
- W1728203356 hasConcept C55493867 @default.
- W1728203356 hasConcept C86803240 @default.
- W1728203356 hasConcept C91523082 @default.
- W1728203356 hasConceptScore W1728203356C104788683 @default.
- W1728203356 hasConceptScore W1728203356C104950815 @default.
- W1728203356 hasConceptScore W1728203356C134651460 @default.
- W1728203356 hasConceptScore W1728203356C147483822 @default.
- W1728203356 hasConceptScore W1728203356C153911025 @default.
- W1728203356 hasConceptScore W1728203356C181199279 @default.
- W1728203356 hasConceptScore W1728203356C185592680 @default.
- W1728203356 hasConceptScore W1728203356C2777320049 @default.
- W1728203356 hasConceptScore W1728203356C2778163477 @default.
- W1728203356 hasConceptScore W1728203356C2778697171 @default.
- W1728203356 hasConceptScore W1728203356C2779123261 @default.
- W1728203356 hasConceptScore W1728203356C54355233 @default.
- W1728203356 hasConceptScore W1728203356C55493867 @default.
- W1728203356 hasConceptScore W1728203356C86803240 @default.
- W1728203356 hasConceptScore W1728203356C91523082 @default.
- W1728203356 hasLocation W17282033561 @default.
- W1728203356 hasLocation W17282033562 @default.
- W1728203356 hasOpenAccess W1728203356 @default.
- W1728203356 hasPrimaryLocation W17282033561 @default.
- W1728203356 hasRelatedWork W1581328433 @default.
- W1728203356 hasRelatedWork W1728203356 @default.
- W1728203356 hasRelatedWork W1974488610 @default.
- W1728203356 hasRelatedWork W1992171999 @default.
- W1728203356 hasRelatedWork W2013662683 @default.
- W1728203356 hasRelatedWork W2021537613 @default.
- W1728203356 hasRelatedWork W2078846694 @default.
- W1728203356 hasRelatedWork W2079114853 @default.
- W1728203356 hasRelatedWork W2089640559 @default.
- W1728203356 hasRelatedWork W80660319 @default.
- W1728203356 isParatext "false" @default.
- W1728203356 isRetracted "false" @default.
- W1728203356 magId "1728203356" @default.
- W1728203356 workType "book-chapter" @default.