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- W1728774443 abstract "An artificial synthetic gene coding for human eIF-4E was cloned into an expression vector and direct expression was attempted in Escherichia coli [BL21(DE3) strain] under the control of T7 promoter. The active gene product which was induced in high yield (ca. 4 mg/ 100 ml) by isopropyl-β-D-thiogalactopyranoside was purified to homogeneity by a two step chromatographic procedure with a good yield (ca. 74%), and was confirmed to be recombinant human eIF-4E by amino acid composition and sequence analyses, isoelectric focusing, and absorption spectral measurements. The identity of three-dimensional structures between the recombinant and native human eIF-4Es was confirmed by CD and fluorescence measurements." @default.
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- W1728774443 date "1994-09-01" @default.
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- W1728774443 title "Direct Expression of a Synthetic Gene in Escherichia coli: Purification and Physicochemical Properties of Human Initiation Factor 4E" @default.
- W1728774443 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a124581" @default.
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