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- W1728954299 abstract "Publisher Summary This article describes the use of a different genetic approach to detect RNA protein-interactions. In this method, termed the three-hybrid system, the binding of a hybrid RNA to each of two hybrid proteins activates transcription of a reporter gene in vivo . The method can be used to detect and analyze RNA-protein interaction when both partners are known or to find a mate when only one is known. One asset of the three-hybrid method, shared with other genetic approaches to finding protein partners, is that a DNA clone of the interacting protein is obtained. With the proliferation of sequence data bases and genomic information, a small bit of sequence information may be enough to help determine whether that protein is a legitimate partner or shed light on its function." @default.
- W1728954299 created "2016-06-24" @default.
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- W1728954299 date "1999-01-01" @default.
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- W1728954299 title "[5] Yeast three-hybrid system to detect and analyze interactions between RNA and protein" @default.
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- W1728954299 doi "https://doi.org/10.1016/s0076-6879(99)06007-3" @default.
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