Matches in SemOpenAlex for { <https://semopenalex.org/work/W1729468081> ?p ?o ?g. }
- W1729468081 abstract "Abstract This unit describes methods for isolating and analyzing rafts by detergent insolubility. To distinguish these rafts from raft‐like membranes isolated by other methods, they are referred to here as detergent‐resistant membranes (DRMs). DRMs can be isolated by flotation on sucrose density gradients or by pelleting after detergent extraction. DRM proteins can be analyzed by SDS‐PAGE and immunoblotting. Additionally, radiolabeled DRM proteins can be analyzed, and lipids can be quantitated by high‐performance thin layer chromatography. Support protocols needed for the lipid analysis are also provided. Finally, protocols for raft disruption by cholesterol removal and measuring the kinetics of such removal are included together with a method that reverses the cholesterol removal (cholesterol repletion)." @default.
- W1729468081 created "2016-06-24" @default.
- W1729468081 creator A5034329109 @default.
- W1729468081 date "2002-10-01" @default.
- W1729468081 modified "2023-09-24" @default.
- W1729468081 title "Isolation and Use of Rafts" @default.
- W1729468081 cites W1498171831 @default.
- W1729468081 cites W1585445496 @default.
- W1729468081 cites W1784927340 @default.
- W1729468081 cites W1966004627 @default.
- W1729468081 cites W1967092440 @default.
- W1729468081 cites W1972145067 @default.
- W1729468081 cites W1980334501 @default.
- W1729468081 cites W1986812906 @default.
- W1729468081 cites W1990927663 @default.
- W1729468081 cites W1998865212 @default.
- W1729468081 cites W2000589601 @default.
- W1729468081 cites W2001217082 @default.
- W1729468081 cites W2012522079 @default.
- W1729468081 cites W2024203551 @default.
- W1729468081 cites W2035465626 @default.
- W1729468081 cites W2050105242 @default.
- W1729468081 cites W2064754325 @default.
- W1729468081 cites W2068504553 @default.
- W1729468081 cites W2072274588 @default.
- W1729468081 cites W2085909518 @default.
- W1729468081 cites W2090272927 @default.
- W1729468081 cites W2121936687 @default.
- W1729468081 cites W2127792983 @default.
- W1729468081 cites W2128963767 @default.
- W1729468081 cites W2130388575 @default.
- W1729468081 cites W2141367941 @default.
- W1729468081 cites W2201941985 @default.
- W1729468081 doi "https://doi.org/10.1002/0471142735.im1110s51" @default.
- W1729468081 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18432868" @default.
- W1729468081 hasPublicationYear "2002" @default.
- W1729468081 type Work @default.
- W1729468081 sameAs 1729468081 @default.
- W1729468081 citedByCount "25" @default.
- W1729468081 countsByYear W17294680812012 @default.
- W1729468081 countsByYear W17294680812013 @default.
- W1729468081 countsByYear W17294680812015 @default.
- W1729468081 countsByYear W17294680812016 @default.
- W1729468081 countsByYear W17294680812017 @default.
- W1729468081 countsByYear W17294680812019 @default.
- W1729468081 countsByYear W17294680812020 @default.
- W1729468081 countsByYear W17294680812021 @default.
- W1729468081 countsByYear W17294680812023 @default.
- W1729468081 crossrefType "journal-article" @default.
- W1729468081 hasAuthorship W1729468081A5034329109 @default.
- W1729468081 hasConcept C121332964 @default.
- W1729468081 hasConcept C144647389 @default.
- W1729468081 hasConcept C148898269 @default.
- W1729468081 hasConcept C15920480 @default.
- W1729468081 hasConcept C178790620 @default.
- W1729468081 hasConcept C185592680 @default.
- W1729468081 hasConcept C2775941552 @default.
- W1729468081 hasConcept C2778163477 @default.
- W1729468081 hasConcept C41625074 @default.
- W1729468081 hasConcept C43617362 @default.
- W1729468081 hasConcept C4725764 @default.
- W1729468081 hasConcept C521977710 @default.
- W1729468081 hasConcept C55493867 @default.
- W1729468081 hasConcept C60644358 @default.
- W1729468081 hasConcept C62520636 @default.
- W1729468081 hasConcept C79266657 @default.
- W1729468081 hasConcept C86803240 @default.
- W1729468081 hasConcept C9132879 @default.
- W1729468081 hasConceptScore W1729468081C121332964 @default.
- W1729468081 hasConceptScore W1729468081C144647389 @default.
- W1729468081 hasConceptScore W1729468081C148898269 @default.
- W1729468081 hasConceptScore W1729468081C15920480 @default.
- W1729468081 hasConceptScore W1729468081C178790620 @default.
- W1729468081 hasConceptScore W1729468081C185592680 @default.
- W1729468081 hasConceptScore W1729468081C2775941552 @default.
- W1729468081 hasConceptScore W1729468081C2778163477 @default.
- W1729468081 hasConceptScore W1729468081C41625074 @default.
- W1729468081 hasConceptScore W1729468081C43617362 @default.
- W1729468081 hasConceptScore W1729468081C4725764 @default.
- W1729468081 hasConceptScore W1729468081C521977710 @default.
- W1729468081 hasConceptScore W1729468081C55493867 @default.
- W1729468081 hasConceptScore W1729468081C60644358 @default.
- W1729468081 hasConceptScore W1729468081C62520636 @default.
- W1729468081 hasConceptScore W1729468081C79266657 @default.
- W1729468081 hasConceptScore W1729468081C86803240 @default.
- W1729468081 hasConceptScore W1729468081C9132879 @default.
- W1729468081 hasIssue "1" @default.
- W1729468081 hasLocation W17294680811 @default.
- W1729468081 hasLocation W17294680812 @default.
- W1729468081 hasOpenAccess W1729468081 @default.
- W1729468081 hasPrimaryLocation W17294680811 @default.
- W1729468081 hasRelatedWork W1591332752 @default.
- W1729468081 hasRelatedWork W2066820623 @default.
- W1729468081 hasRelatedWork W2075289114 @default.
- W1729468081 hasRelatedWork W2082179247 @default.
- W1729468081 hasRelatedWork W2123413533 @default.
- W1729468081 hasRelatedWork W2147645103 @default.
- W1729468081 hasRelatedWork W2560070108 @default.
- W1729468081 hasRelatedWork W3004169832 @default.
- W1729468081 hasRelatedWork W4287899158 @default.