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- W173031013 abstract "Next-generation sequencing technologies for whole-genome sequencing of mycobacteria are rapidly becoming an attractive alternative to more traditional sequencing methods. In particular this technology is proving useful for genome-wide identification of mutations in mycobacteria (comparative genomics) as well as for de novo assembly of whole genomes. Next-generation sequencing however generates a vast quantity of data that can only be transformed into a usable and comprehensible form using bioinformatics. Here we describe the methodology one would use to prepare libraries for whole-genome sequencing, and the basic bioinformatics to identify mutations in a genome following Illumina HiSeq or MiSeq sequencing, as well as de novo genome assembly following sequencing using Pacific Biosciences (PacBio)." @default.
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- W173031013 date "2015-01-01" @default.
- W173031013 modified "2023-10-03" @default.
- W173031013 title "Whole-Genome Sequencing for Comparative Genomics and De Novo Genome Assembly" @default.
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- W173031013 doi "https://doi.org/10.1007/978-1-4939-2450-9_1" @default.
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