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- W1734157281 abstract "This chapter describes modifications of the standard techniques used for either protein kinase characterization or gene regulation studies with emphasis on the unique possibilities and pitfalls that are observed when manipulating a multisubunit enzyme system by gene transfer techniques. In utilizing the techniques, it is important to fully appreciate the complex regulatory network in which the cAMP-dependent protein kinases function. For example, the overexpression of exogenous C subunit has led to a compensatory increase in regulatory subunits in several cell types. The chapter also describe four basic parts of eukaryotic expression vectors: (1) a transcriptional promoter sequence, (2) the structural gene coding for the protein which is to be expressed, (3) a eukaryotic poly(A) addition signal sequence, and (4) a prokaryotic replication/antibiotic resistance region for growth of large amounts of the expression vector in bacteria. Expression vectors may also contain intron sequences that must be removed by RNA splicing to produce a functional mRNA but these are not essential in most cases and are not included in the protein kinase vectors." @default.
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- W1734157281 date "1988-01-01" @default.
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- W1734157281 title "[28] Application of molecular genetic techniques to the cAMP-dependent protein kinase system" @default.
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- W1734157281 doi "https://doi.org/10.1016/0076-6879(88)59030-4" @default.
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