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• W1736095737 abstract "Studies showed that membrane tubulin (but not brain tubulin) could be reincorporated into membranes of uniform buoyant density when the solubilizing detergent was removed. It was demonstrated by a number of approaches that the tubulin and associated minor proteins formed a large micellar complex with lipids and detergent on partial delipidation. Gentle delipidation with low concentrations of detergent suggests that membrane tubulin and associated proteins form a discrete “membrane skeleton” surrounding the axoneme. Tubulin has also been described as a prominent constituent of frog olfactory and of bovine retinal rod outer segment plasma but not disk membranes. Such observations, coupled with the consistent appearance of tubulin in mechanically sensitive cilia and in various neuronal tissues, have led to suggestions that membrane tubulin may serve some structural or functional role in sensory membranes. Tubulin is the major constituent of embryonic cilia in sea urchin embryos, whereas, in scallop cilia, it is lipid associated and can be recycled in vitro into membranes. Pulse-chase studies have demonstrated that this tubulin most likely is the precursor to the tubulin of the assembling axoneme. Most other newly synthesized axonemal protein are not found associated with the tubulin-rich detergent-solubilized membrane/matrix fraction but, rather, are associated with the axoneme proper, suggesting two separate or separable pathways for tubulin and lipids, on one hand, and the various structural proteins of the axoneme, on the other. The presence of “membrane” tubulin might be a measure of tubulin and lipid on the move. The chapter discusses preparation of membrane tubulin by reconstitution. This procedure uses in vitro membrane reconstitution to separate membrane tubulin and its associated proteins from soluble proteins of the axonemal matrix. The solutions needed and the procedure has been discussed in detail. There are some purification methods discussed—isopycnic centrifugation and Triton X-114 delipidation." @default.
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• W1736095737 date "1995-01-01" @default.
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• W1736095737 title "Chapter 61 Ciliary Membrane Tubulin: Isolation and Fractionation" @default.
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• W1736095737 doi "https://doi.org/10.1016/s0091-679x(08)60842-0" @default.
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